Literature summary for 1.7.1.3 extracted from

Takasaki, K.; Shoun, H.; Nakamura, A.; Hoshino, T.; Takaya, N.
Unusual transcription regulation of the niaD gene under anaeobic conditions supporting fungal ammonia fermentation (2004), Biosci. Biotechnol. Biochem., 68, 978-980.

Search for more literature for 1.7.1.3

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	exogenous nitrate induces the enzyme expression, can be repressed by ammonium under aerobic conditions, but not under anaerobic conditions, promotor activity study	Aspergillus nidulans

Cloned(Commentary)

Cloned (Comment)	Organism
construction of a fusion gene comprising gene the promotor of gene niaD and the Escherichia coli beta-glucuronidase gene, i.e. GUS, for analysis of promotor activity in Aspergillus nidulans	Aspergillus nidulans

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of null-mutants of gene niaD	Aspergillus nidulans

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	exogenous ammonium represses enzyme expression under oxic conditions in presence or absence of nitrate, while under anoxic conditions the enzyme is expressed even in the presence of ammonium, promotor activity study	Aspergillus nidulans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
nitrate + NADPH	Aspergillus nidulans	enzyme is essential for reduction of nitrate to ammonia, under oxic conditions the enzyme is involved in nitrate assimilation, under anoxic conditions the enzyme is used for dissimilatory nitrate reduction, transcription regulation mechanism via ammonium, nitrate, and O2 concentrations, overview	nitrite + NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Aspergillus nidulans	-	gene niaD, several strains	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0001	-	below, wild-type enzyme, aerobic conditions, presence of ammonium	Aspergillus nidulans
0.0001	-	below, wild-type enzyme, aerobic conditions, presence of nitrate and ammonium	Aspergillus nidulans
0.012	-	wild-type enzyme, anaerobic conditions, presence of ammonium	Aspergillus nidulans
0.013	-	wild-type enzyme, aerobic conditions, presence of nitrate	Aspergillus nidulans
0.018	-	wild-type enzyme, anaerobic conditions, presence of nitrate	Aspergillus nidulans
0.019	-	wild-type enzyme, anaerobic conditions, presence of nitrate and ammonium	Aspergillus nidulans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
nitrate + NADPH	-	Aspergillus nidulans	nitrite + NADP+	-	?
nitrate + NADPH	enzyme is essential for reduction of nitrate to ammonia, under oxic conditions the enzyme is involved in nitrate assimilation, under anoxic conditions the enzyme is used for dissimilatory nitrate reduction, transcription regulation mechanism via ammonium, nitrate, and O2 concentrations, overview	Aspergillus nidulans	nitrite + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
nitrate reductase	-	Aspergillus nidulans

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Aspergillus nidulans

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Release 2023.1 (January 2023)