BRENDA - Enzyme Database show
show all sequences of 1.7.1.2

Structure and properties of assimilatory nitrate reductase from the yeast Candida nitratophila

Hipkin, C.R.; Ali, A.H.; Cannons, A.; J. Gen. Microbiol. 132, 1997-2003 (1986)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
Labile to dilution with protein-free buffer, 2.5 mg bovine serum albumin, stabilizes
Candida nitratophila
Inhibitors
Inhibitors
Commentary
Organism
Structure
cyanide
preincubation with NAD(P)H and cyanide: inactivation
Candida nitratophila
NAD(P)H
preincubation with NAD(P)H alone: activation; preincubation with NAD(P)H and cyanide: inactivation
Candida nitratophila
p-chloromercuribenzoate
-
Candida nitratophila
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.017
-
NADH
-
Candida nitratophila
0.03
-
NADPH
-
Candida nitratophila
0.11
-
nitrate
cosubstrate NADPH
Candida nitratophila
0.12
-
nitrate
cofactor NADH
Candida nitratophila
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
95000
-
4 * 95000, Candida nitratophila, SDS-PAGE
Candida nitratophila
365000
-
Candida nitratophila, sedimentation equilibrium centrifugation
Candida nitratophila
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Candida nitratophila
-
-
-
Purification (Commentary)
Commentary
Organism
-
Candida nitratophila
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
217.4
-
-
Candida nitratophila
Storage Stability
Storage Stability
Organism
4°C, 50% loss of activity after 7 days
Candida nitratophila
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NAD(P)H + nitrate
-
208084
Candida nitratophila
NAD(P)+ + nitrite + H2O
-
208084
Candida nitratophila
-
Subunits
Subunits
Commentary
Organism
tetramer
4 * 95000, Candida nitratophila, SDS-PAGE
Candida nitratophila
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
above 6000
Candida nitratophila
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome b557
contains cytochrome b557
Candida nitratophila
FAD
maximum activity requires addition of flavin nucleotides, FAD more effective than FMN
Candida nitratophila
FMN
maximum activity requires addition of flavin nucleotides, FAD is more effective than FMN
Candida nitratophila
NADH
preincubation with NAD(P)H and CN- causes inactivation, preincubation with NAD(P)H activates; utilizes both NADH and NADPH, more active with NADH
Candida nitratophila
NADPH
preincubation with NAD(P)H and CN- causes inactivation, preincubation with NAD(P)H alone activates; utilizes both NADH and NADPH, more active with NADH
Candida nitratophila
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome b557
contains cytochrome b557
Candida nitratophila
FAD
maximum activity requires addition of flavin nucleotides, FAD more effective than FMN
Candida nitratophila
FMN
maximum activity requires addition of flavin nucleotides, FAD is more effective than FMN
Candida nitratophila
NADH
preincubation with NAD(P)H and CN- causes inactivation, preincubation with NAD(P)H activates; utilizes both NADH and NADPH, more active with NADH
Candida nitratophila
NADPH
preincubation with NAD(P)H and CN- causes inactivation, preincubation with NAD(P)H alone activates; utilizes both NADH and NADPH, more active with NADH
Candida nitratophila
General Stability (protein specific)
General Stability
Organism
Labile to dilution with protein-free buffer, 2.5 mg bovine serum albumin, stabilizes
Candida nitratophila
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
cyanide
preincubation with NAD(P)H and cyanide: inactivation
Candida nitratophila
NAD(P)H
preincubation with NAD(P)H alone: activation; preincubation with NAD(P)H and cyanide: inactivation
Candida nitratophila
p-chloromercuribenzoate
-
Candida nitratophila
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.017
-
NADH
-
Candida nitratophila
0.03
-
NADPH
-
Candida nitratophila
0.11
-
nitrate
cosubstrate NADPH
Candida nitratophila
0.12
-
nitrate
cofactor NADH
Candida nitratophila
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
95000
-
4 * 95000, Candida nitratophila, SDS-PAGE
Candida nitratophila
365000
-
Candida nitratophila, sedimentation equilibrium centrifugation
Candida nitratophila
Purification (Commentary) (protein specific)
Commentary
Organism
-
Candida nitratophila
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
217.4
-
-
Candida nitratophila
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 50% loss of activity after 7 days
Candida nitratophila
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NAD(P)H + nitrate
-
208084
Candida nitratophila
NAD(P)+ + nitrite + H2O
-
208084
Candida nitratophila
-
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 95000, Candida nitratophila, SDS-PAGE
Candida nitratophila
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
above 6000
Candida nitratophila
Other publictions for EC 1.7.1.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
697599
Sempruch
-
Purification and some physioch ...
Triticale sp.
Electron. J. Pol. Agric. Univ.
11
1-1
2008
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1
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5
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2
1
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672297
Morozkina
Isolation, purification, and c ...
Rhodotorula glutinis
Biochemistry (Moscow)
70
809-814
2005
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3
1
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1
2
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1
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1
1
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1
1
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676409
Eckardt
-
Moco mojo: Crystal structure r ...
Komagataella pastoris
Plant Cell
17
1029-1031
2005
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1
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658230
Ferreyra
An amperometric nitrate reduct ...
Aspergillus niger
Bioelectrochemistry
64
61-70
2004
-
1
-
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1
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1
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658241
Ahmad
-
Relationship between soil nitr ...
Brassica juncea
Biol. Plant.
46
295-296
2003
-
-
-
-
-
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-
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1
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1
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1
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-
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-
-
658464
Dziedzic
Comparison of cadmium and enzy ...
Aspergillus niger
Clin. Chim. Acta
335
65-74
2003
-
1
-
-
-
-
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1
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1
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-
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-
658564
Bhushan
Biotransformation of hexahydro ...
Aspergillus niger
Environ. Sci. Technol.
36
3104-3108
2002
-
1
-
-
-
-
-
-
-
-
-
-
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1
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1
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1
-
1
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1
-
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-
-
-
680437
EBERL
Inactivation of gltB abolishes ...
Pseudomonas putida, Pseudomonas putida KT 2240
J. Bacteriol.
182
3368-3376
2000
-
-
-
-
-
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-
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-
6
-
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208086
Hipkin
-
Physical and chemical properti ...
Candida nitratophila
Biochem. Soc. Trans.
17
928-929
1989
-
-
-
-
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1
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1
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1
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1
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3
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3
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1
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1
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-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
208085
Deising
-
Nitrate reductase from Sphagnu ...
Sphagnum cuspidatum, Sphagnum fallax, Sphagnum fimbriatum, Sphagnum magellanicum, Sphagnum molle, Sphagnum palustre, Sphagnum papillosum, Sphagnum rubellum, Sphagnum squarrosum, Sphagnum subnitens, Sphagnum subsecundum
Z. Naturforsch. C
42
653-656
1987
-
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11
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11
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12
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11
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12
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208084
Hipkin
-
Structure and properties of as ...
Candida nitratophila
J. Gen. Microbiol.
132
1997-2003
1986
-
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1
3
4
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2
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1
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1
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1
1
1
1
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1
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5
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5
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1
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3
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4
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1
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1
1
1
1
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1
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-
208097
Harker
-
NAD(P)H bispecific nitrate red ...
Hordeum vulgare
Phytochemistry
25
1275-1279
1986
-
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2
1
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1
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208088
Minagawa
-
The in vivo inactivation and r ...
Wickerhamomyces anomalus
Agric. Biol. Chem.
49
2217-2219
1985
-
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1
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1
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1
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2
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208089
Minagawa
-
The in vitro irreversible inac ...
Wickerhamomyces anomalus
Agric. Biol. Chem.
49
2213-2215
1985
-
-
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-
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1
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1
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208092
Cordoba
-
Role of the diaphorase moiety ...
Chlamydomonas reinhardtii
Biochim. Biophys. Acta
827
8-13
1985
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1
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208093
Streit
Nitrate reductases from wild-t ...
Glycine max
Plant Physiol.
78
80-84
1985
-
-
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4
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1
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1
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1
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1
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208098
Lopez-Ruiz
Nitrate reductase of green alg ...
Chlorolobion braunii
Plant Physiol.
78
614-618
1985
-
-
-
-
-
-
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1
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1
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1
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208087
Minagawa
-
Assimilatory nitrate reductase ...
Wickerhamomyces anomalus
Agric. Biol. Chem.
47
125-127
1983
-
-
-
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2
1
1
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-
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1
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1
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1
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1
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4
-
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4
-
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-
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-
2
1
1
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1
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1
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1
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208099
Marquez
-
Studies by affinity chromatogr ...
Ankistrodesmus braunii
J. Chromatogr.
235
435-443
1982
-
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208100
De La Rosa
-
Dissociation of FAD from the N ...
Ankistrodesmus braunii
Z. Naturforsch. C
37
24-30
1982
-
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1
1
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1
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1
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1
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208091
Fernandez
In vitro complementation of as ...
Chlamydomonas reinhardtii
Biochim. Biophys. Acta
657
1-12
1981
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208101
De La Rosa
Composition and structure of a ...
Ankistrodesmus braunii
J. Biol. Chem.
256
5814-5819
1981
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4
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1
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208103
Guerrero
-
The assimilatory nitrate-reduc ...
Ankistrodesmus braunii, Rhodotorula glutinis
Annu. Rev. Plant Physiol.
32
169-204
1981
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2
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2
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2
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208094
Orihuel-Iranzo
Development of NAD(P)H: and NA ...
Glycine max
Plant Physiol.
65
595-599
1980
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208102
De La Rosa
Purification and properties of ...
Ankistrodesmus braunii
Eur. J. Biochem.
106
249-256
1980
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1
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208090
Hipkin
Pyridine nucleotide specificit ...
Chlorella variegata
Planta
144
137-141
1979
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3
4
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208095
Guerrero
Purification and properties of ...
Rhodotorula glutinis
Biochim. Biophys. Acta
482
272-285
1977
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5
3
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208096
Rigano
Studies on nitrate reductase f ...
Cyanidium caldarium
Arch. Mikrobiol.
76
265-276
1971
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