Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Rhodococcus opacus |
General Stability | Organism |
---|---|
non-immobilized enzyme shows a constant decrease in activity to become entirely inactive after 35 days, unlike activities of the immobilized biocatalysts (meso-cellular foams and mesoporous silica) which decline significantly during the first week and then they stabilize to retain ca. 27%-42% of the initial enzyme activity after 63 days | Rhodococcus opacus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus opacus | - |
- |
- |
Rhodococcus opacus 1CP | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Allura Red + NADH + H+ | substrate for immobilized enzyme (catalyst meso-cellular foam-A) only | Rhodococcus opacus | ? | - |
? | |
Allura Red + NADH + H+ | substrate for immobilized enzyme (catalyst meso-cellular foam-A) only | Rhodococcus opacus 1CP | ? | - |
? | |
Brilliant Black + NADH + H+ | low activity | Rhodococcus opacus | ? | - |
? | |
Brilliant Black + NADH + H+ | low activity | Rhodococcus opacus 1CP | ? | - |
? | |
methyl orange + NADH + H+ | substrate for immobilized enzyme (catalyst meso-cellular foam-A) only | Rhodococcus opacus | ? | - |
? | |
methyl orange + NADH + H+ | substrate for immobilized enzyme (catalyst meso-cellular foam-A) only | Rhodococcus opacus 1CP | ? | - |
? | |
methyl red + NADH + H+ | substrate for immobilized enzyme (catalyst meso-cellular foam-A) only | Rhodococcus opacus | ? | - |
? | |
methyl red + NADH + H+ | substrate for immobilized enzyme (catalyst meso-cellular foam-A) only | Rhodococcus opacus 1CP | ? | - |
? | |
additional information | free enzyme has no activity with azobenzene, 4-phenylazophenol, Allura Red, Amido Black, Reactive Orange, Para Red, Chlorazol Black and Methyl Orange | Rhodococcus opacus | ? | - |
- |
|
additional information | free enzyme has no activity with azobenzene, 4-phenylazophenol, Allura Red, Amido Black, Reactive Orange, Para Red, Chlorazol Black and Methyl Orange | Rhodococcus opacus 1CP | ? | - |
- |
|
Reactive Orange + NADH + H+ | substrate for immobilized enzyme (catalyst meso-cellular foam-A) only | Rhodococcus opacus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25000, SDS-PAGE | Rhodococcus opacus |
Synonyms | Comment | Organism |
---|---|---|
azoreductase | - |
Rhodococcus opacus |
AzoRo | - |
Rhodococcus opacus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Rhodococcus opacus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Rhodococcus opacus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
the mesoporous silicas-immobilized enzyme is active after incubation at pH 4.0 for 60 h | Rhodococcus opacus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Rhodococcus opacus |