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Literature summary for 1.7.1.17 extracted from

  • Verma, K.; Kundu, D.; Kundu, L.M.; Singh, A.K.; Dubey, V.K.
    Folding and stability of recombinant azoreductase enzyme from Chromobacterium violaceum (2019), Enzyme Microb. Technol., 131, 109433 .
    View publication on PubMed

General Stability

General Stability Organism
50% of azoreductase is unfolded up to 2 M urea and complete denaturation stage is up to 4 M urea concentration, respectively. 55% of azoreductase is unfolded up to 2 M GdmCl condition Chromobacterium violaceum

Inhibitors

Inhibitors Comment Organism Structure
guanidine hydrochloride 55% activity of azoreductase decreases at up to 2 M guanidine hydrochloride concentration while complete loss of activity is found at 3.5 M concentration Chromobacterium violaceum
Urea 50% activity is lost up to 2 M urea concentration and complete loss of activity is observed at 4 M urea Chromobacterium violaceum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
gel filtration Chromobacterium violaceum

Organism

Organism UniProt Comment Textmining
Chromobacterium violaceum Q7NWH3
-
-
Chromobacterium violaceum ATCC 12472 Q7NWH3
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Chromobacterium violaceum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amaranth + 2 NADH + 2 H+
-
Chromobacterium violaceum 4-aminonaphthalene-1-sulfonic acid + 4-amino-3-hydroxynaphthalene-2,7-disulfonic acid + 2 NAD+
-
?
amaranth + 2 NADH + 2 H+
-
Chromobacterium violaceum ATCC 12472 4-aminonaphthalene-1-sulfonic acid + 4-amino-3-hydroxynaphthalene-2,7-disulfonic acid + 2 NAD+
-
?
methyl orange + 2 NADH + 2 H+
-
Chromobacterium violaceum ?
-
?
methyl orange + 2 NADH + 2 H+
-
Chromobacterium violaceum ATCC 12472 ?
-
?
methyl red + 2 NADH + 2 H+
-
Chromobacterium violaceum ?
-
?
methyl red + 2 NADH + 2 H+
-
Chromobacterium violaceum ATCC 12472 ?
-
?

Subunits

Subunits Comment Organism
homodimer x * 24000, SDS-PAGE Chromobacterium violaceum

Synonyms

Synonyms Comment Organism
FMN dependent azoreductase
-
Chromobacterium violaceum

Cofactor

Cofactor Comment Organism Structure
FMN FMN is required for folding, stability and activity Chromobacterium violaceum
NADH
-
Chromobacterium violaceum