General Stability | Organism |
---|---|
50% of azoreductase is unfolded up to 2 M urea and complete denaturation stage is up to 4 M urea concentration, respectively. 55% of azoreductase is unfolded up to 2 M GdmCl condition | Chromobacterium violaceum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
guanidine hydrochloride | 55% activity of azoreductase decreases at up to 2 M guanidine hydrochloride concentration while complete loss of activity is found at 3.5 M concentration | Chromobacterium violaceum | |
Urea | 50% activity is lost up to 2 M urea concentration and complete loss of activity is observed at 4 M urea | Chromobacterium violaceum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
gel filtration | Chromobacterium violaceum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chromobacterium violaceum | Q7NWH3 | - |
- |
Chromobacterium violaceum ATCC 12472 | Q7NWH3 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Chromobacterium violaceum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amaranth + 2 NADH + 2 H+ | - |
Chromobacterium violaceum | 4-aminonaphthalene-1-sulfonic acid + 4-amino-3-hydroxynaphthalene-2,7-disulfonic acid + 2 NAD+ | - |
? | |
amaranth + 2 NADH + 2 H+ | - |
Chromobacterium violaceum ATCC 12472 | 4-aminonaphthalene-1-sulfonic acid + 4-amino-3-hydroxynaphthalene-2,7-disulfonic acid + 2 NAD+ | - |
? | |
methyl orange + 2 NADH + 2 H+ | - |
Chromobacterium violaceum | ? | - |
? | |
methyl orange + 2 NADH + 2 H+ | - |
Chromobacterium violaceum ATCC 12472 | ? | - |
? | |
methyl red + 2 NADH + 2 H+ | - |
Chromobacterium violaceum | ? | - |
? | |
methyl red + 2 NADH + 2 H+ | - |
Chromobacterium violaceum ATCC 12472 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x * 24000, SDS-PAGE | Chromobacterium violaceum |
Synonyms | Comment | Organism |
---|---|---|
FMN dependent azoreductase | - |
Chromobacterium violaceum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | FMN is required for folding, stability and activity | Chromobacterium violaceum | |
NADH | - |
Chromobacterium violaceum |