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Literature summary for 1.7.1.17 extracted from

  • Misal, S.A.; Lingojwar, D.P.; Lokhande, M.N.; Lokhande, P.D.; Gawai, K.R.
    Enzymatic transformation of nitro-aromatic compounds by a flavin-free NADH azoreductase from Lysinibacillus sphaericus (2014), Biotechnol. Lett., 36, 127-131 .
    View publication on PubMed
Search for more literature for 1.7.1.17

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 NADH pH 7.4, 37°C Lysinibacillus sphaericus
0.034
-
 Amaranth pH 7.4, 37°C Lysinibacillus sphaericus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 gel filtration and PAGE Lysinibacillus sphaericus

Organism

Organism UniProt Comment Textmining
Lysinibacillus sphaericus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lysinibacillus sphaericus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18
-
 substrate orange G, cofactor NADH, pH 7.4, 37°C Lysinibacillus sphaericus
22
-
 substrate amaranth, cofactor NADPH, pH 7.4, 37°C Lysinibacillus sphaericus
25
-
 substrate amaranth, cofactor FADH2, pH 7.4, 37°C Lysinibacillus sphaericus
26
-
 substrate amaranth, cofactor FMNH2, pH 7.4, 37°C Lysinibacillus sphaericus
63
-
 substrate fast red E, cofactor NADH, pH 7.4, 37°C Lysinibacillus sphaericus
74
-
 substrate amaranth, cofactor NADH, pH 7.4, 37°C Lysinibacillus sphaericus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amaranth + 2 NADH + 2 H+
-
 Lysinibacillus sphaericus 4-aminonaphthalene-1-sulfonic acid + 4-amino-3-hydroxynaphthalene-2,7-disulfonic acid + 2 NAD+
-
 ?
amaranth + 2 NADPH + 2 H+
-
 Lysinibacillus sphaericus 4-aminonaphthalene-1-sulfonic acid + 4-amino-3-hydroxynaphthalene-2,7-disulfonic acid + 2 NADP+
-
 ?
fast red E + 2 NADH + 2 H+
-
 Lysinibacillus sphaericus 4-aminonaphthalene-1-sulfonic acid + 5-amino-6-hydroxynaphthalene-2-sulfonic acid + 2 NAD+
-
 ?
fast red E + 2 NADPH + 2 H+
-
 Lysinibacillus sphaericus 4-aminonaphthalene-1-sulfonic acid + 5-amino-6-hydroxynaphthalene-2-sulfonic acid + 2 NADP+
-
 ?
additional information enzyme additionally acts as nitroreductase and selectively reduces the nitro group of 2-nitrophenol, 4-nitrobenzoic acid, 2-nitro-benzaldehyde and 3-nitrophenol Lysinibacillus sphaericus ?
-
 ?
orange G + 2 NADH + 2 H+
-
 Lysinibacillus sphaericus aniline + 8-amino-7-hydroxynaphthalene-1,3-disulfonic acid + 2 NAD+
-
 ?
orange G + 2 NADPH + 2 H+
-
 Lysinibacillus sphaericus aniline + 8-amino-7-hydroxynaphthalene-1,3-disulfonic acid + 2 NADP+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 29000, SDS-PAGE Lysinibacillus sphaericus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
 Lysinibacillus sphaericus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 stable for 30 min Lysinibacillus sphaericus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Lysinibacillus sphaericus

Cofactor

Cofactor Comment Organism Structure
FADH2
-
 Lysinibacillus sphaericus
FMNH2
-
 Lysinibacillus sphaericus
NADH NADH is the preferred cofactor Lysinibacillus sphaericus
NADPH
-
 Lysinibacillus sphaericus