Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
gel fitlration | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | Q9X4K2 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25.8 | - |
30°C, pH 7.4, substrate 4'-(dimethylamino)-azobenzene-2-carboxylic acid | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4'-(diethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+ | i.e. ethyl red | Geobacillus stearothermophilus | 2-aminobenzoic acid + N,N-diethyl-p-phenylenediamine + 2 NAD+ | - |
? | |
4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+ | i.e. methyl red | Geobacillus stearothermophilus | 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+ | - |
? | |
acid red 88 + 2 NADH + 2 H+ | - |
Geobacillus stearothermophilus | 4-aminobenzene-1-sulfonic acid + 1-aminonaphthalen-2-ol + 2 NAD+ | - |
? | |
congo red + 2 NADH + 2 H+ | - |
Geobacillus stearothermophilus | ? + 2 NAD+ | - |
? | |
orange I + 2 NADH + 2 H+ | - |
Geobacillus stearothermophilus | 4-aminobenzene-1-sulfonic acid + 4-aminonaphthalen-1-ol + 2 NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 23000, SDS-PAGE | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
AzoR | - |
Geobacillus stearothermophilus |
AzrG | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
stable for 1 month | Geobacillus stearothermophilus |
65 | - |
stable for 1 h | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Geobacillus stearothermophilus | |
NADH | reaction with NADH is 13fold faster than with NADPH | Geobacillus stearothermophilus | |
NADPH | reaction with NADH is 13fold faster than with NADPH | Geobacillus stearothermophilus |