Literature summary for 1.7.1.17 extracted from

Matsumoto, K.; Mukai, Y.; Ogata, D.; Shozui, F.; Nduko, J.M.; Taguchi, S.; Ooi, T.
Characterization of thermostable FMN-dependent NADH azoreductase from the moderate thermophile Geobacillus stearothermophilus (2010), Appl. Microbiol. Biotechnol., 86, 1431-1438 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48000	-	gel fitlration	Geobacillus stearothermophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	Q9X4K2	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
25.8	-	30°C, pH 7.4, substrate 4'-(dimethylamino)-azobenzene-2-carboxylic acid	Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4'-(diethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+	i.e. ethyl red	Geobacillus stearothermophilus	2-aminobenzoic acid + N,N-diethyl-p-phenylenediamine + 2 NAD+	-	?
4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+	i.e. methyl red	Geobacillus stearothermophilus	2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+	-	?
acid red 88 + 2 NADH + 2 H+	-	Geobacillus stearothermophilus	4-aminobenzene-1-sulfonic acid + 1-aminonaphthalen-2-ol + 2 NAD+	-	?
congo red + 2 NADH + 2 H+	-	Geobacillus stearothermophilus	? + 2 NAD+	-	?
orange I + 2 NADH + 2 H+	-	Geobacillus stearothermophilus	4-aminobenzene-1-sulfonic acid + 4-aminonaphthalen-1-ol + 2 NAD+	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 23000, SDS-PAGE	Geobacillus stearothermophilus

Synonyms

Synonyms	Comment	Organism
AzoR	-	Geobacillus stearothermophilus
AzrG	-	Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
85	-	-	Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
30	-	stable for 1 month	Geobacillus stearothermophilus
65	-	stable for 1 h	Geobacillus stearothermophilus

Cofactor

Cofactor	Comment	Organism	Structure
FMN	-	Geobacillus stearothermophilus
NADH	reaction with NADH is 13fold faster than with NADPH	Geobacillus stearothermophilus
NADPH	reaction with NADH is 13fold faster than with NADPH	Geobacillus stearothermophilus

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Release 2023.1 (January 2023)