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Literature summary for 1.7.1.16 extracted from
- Nitroreductase from Salmonella typhimurium: characterization and catalytic activity (2010), Org. Biomol. Chem., 8, 1826-1832.
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | P15888 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.1 | - |
substrate nitrofurazone, pH 7.2, 20°C | Salmonella enterica subsp. enterica serovar Typhimurium |
| 13.6 | - |
substrate nitrofurazone, pH 7.5, 45°C | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme shows broad substrate acceptance for various nitro compounds such as 1-nitrocyclohexene and aliphatic nitroalkenes (alkene reductase activity), as well as nitrobenzene (nitroreductase activity), with substrate conversion efficiency of >95% | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? |  |
| nitrobenzene + 2 NADPH + 2 H+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | aniline + 2 NADP+ + H2O | reduction of nitrobenzene to aniline occurs through the formation of nitrosobenzene and phenylhydroxylamine as intermediates. More than 99% overall conversion | ? | |
| nitrofurazone + 2 NADPH + 2 H+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? + 2 NADP+ + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Oxygen-insensitive NAD(P)H nitroreductase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
half-life 365 h | Salmonella enterica subsp. enterica serovar Typhimurium |
| 45 | - |
half-life 17.9 h | Salmonella enterica subsp. enterica serovar Typhimurium |
| 50 | - |
half-life 2.3 h | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
considerable loss of activity above | Salmonella enterica subsp. enterica serovar Typhimurium |
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