Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | 1 mM, no residual activity | Gluconobacter oxydans | |
glutathione | 1 mM, 41% residual activity | Gluconobacter oxydans | |
menadione | 1 mM, 61% residual activity | Gluconobacter oxydans | |
Sodium azide | 1 mM, 52% residual activity | Gluconobacter oxydans | |
Triton X-100 | 1% w/v, 84% residual activity | Gluconobacter oxydans | |
Tween 20 | 1% w/v, 64% residual activity | Gluconobacter oxydans | |
ZnSO4 | 1 mM, no residual activity | Gluconobacter oxydans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
(2E)-2-[(5-nitrofuran-2-yl)methylidene]hydrazine-1-carboxamide | pH 8.0, 37°C | Gluconobacter oxydans | |
0.054 | - |
5-(aziridin-1-yl)-2,4-dinitrobenzamide | pH 8.0, 37°C | Gluconobacter oxydans | |
0.128 | - |
1,3-dinitrobenzene | pH 8.0, 37°C | Gluconobacter oxydans | |
0.153 | - |
NADPH | pH 8.0, 37°C | Gluconobacter oxydans | |
0.264 | - |
3-nitrotoluene | pH 8.0, 37°C | Gluconobacter oxydans | |
0.282 | - |
4-nitrotoluene | pH 8.0, 37°C | Gluconobacter oxydans | |
0.309 | - |
4-chloronitrobenzene | pH 8.0, 37°C | Gluconobacter oxydans | |
0.468 | - |
2-chloronitrobenzene | pH 8.0, 37°C | Gluconobacter oxydans | |
0.545 | - |
nitrobenzene | pH 8.0, 37°C | Gluconobacter oxydans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | Q5FSN8 | - |
- |
Gluconobacter oxydans 621H | Q5FSN8 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11000 | - |
substrate nitrobenzene, pH 8.0, 37°C | Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E)-2-[(5-nitrofuran-2-yl)methylidene]hydrazine-1-carboxamide + 2 NADPH + 2 H+ | i.e. anti-infective furacilin | Gluconobacter oxydans | (2E)-2-[[5-(hydroxyamino)furan-2-yl]methylidene]hydrazine-1-carboxamide + 2 NADP+ | - |
? | |
(2E)-2-[(5-nitrofuran-2-yl)methylidene]hydrazine-1-carboxamide + 2 NADPH + 2 H+ | i.e. anti-infective furacilin | Gluconobacter oxydans 621H | (2E)-2-[[5-(hydroxyamino)furan-2-yl]methylidene]hydrazine-1-carboxamide + 2 NADP+ | - |
? | |
1,3-dinitrobenzene + 2 NADPH + 2 H+ | - |
Gluconobacter oxydans | ? + 2 NADP+ | - |
? | |
1,3-dinitrobenzene + 2 NADPH + 2 H+ | - |
Gluconobacter oxydans 621H | ? + 2 NADP+ | - |
? | |
2-chloronitrobenzene + 2 NADPH + 2 H+ | - |
Gluconobacter oxydans | 2-chloro-N-hydroxyaniline + 2 NADP+ + H2O | - |
? | |
2-chloronitrobenzene + 2 NADPH + 2 H+ | - |
Gluconobacter oxydans 621H | 2-chloro-N-hydroxyaniline + 2 NADP+ + H2O | - |
? | |
3-nitrotoluene + 2 NADPH + 2 H+ | - |
Gluconobacter oxydans | N-hydroxy-3-methylaniline + 2 NADP+ + H2O | - |
? | |
3-nitrotoluene + 2 NADPH + 2 H+ | - |
Gluconobacter oxydans 621H | N-hydroxy-3-methylaniline + 2 NADP+ + H2O | - |
? | |
4-chloronitrobenzene + 2 NADPH + 2 H+ | - |
Gluconobacter oxydans | 4-chloro-N-hydroxyaniline + 2 NADP+ + H2O | - |
? | |
4-nitrotoluene + 2 NADPH + 2 H+ | - |
Gluconobacter oxydans | N-hydroxy-4-methylaniline + 2 NADP+ + H2O | - |
? | |
5-(aziridin-1-yl)-2,4-dinitrobenzamide + 2 NADPH + 2 H+ | - |
Gluconobacter oxydans | ? + 2 NADP+ | enzyme activates the dinitrobenzamide cancer prodrug CB1954, i.e. 5-(aziridin-1-yl)-2,4-dinitrobenzamide | ? | |
nitrobenzene + 2 NADPH + 2 H+ | - |
Gluconobacter oxydans | N-hydroxyaniline + 2 NADP+ + H2O | - |
? | |
nitrobenzene + 2 NADPH + 2 H+ | - |
Gluconobacter oxydans 621H | N-hydroxyaniline + 2 NADP+ + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
(2E)-2-[(5-nitrofuran-2-yl)methylidene]hydrazine-1-carboxamide | pH 8.0, 37°C | Gluconobacter oxydans | |
0.12 | - |
4-chloronitrobenzene | pH 8.0, 37°C | Gluconobacter oxydans | |
0.13 | - |
4-nitrotoluene | pH 8.0, 37°C | Gluconobacter oxydans | |
0.15 | - |
2-chloronitrobenzene | pH 8.0, 37°C | Gluconobacter oxydans | |
0.18 | - |
nitrobenzene | pH 8.0, 37°C | Gluconobacter oxydans | |
0.18 | - |
3-nitrotoluene | pH 8.0, 37°C | Gluconobacter oxydans | |
0.24 | - |
1,3-dinitrobenzene | pH 8.0, 37°C | Gluconobacter oxydans | |
1.05 | - |
NADPH | pH 8.0, 37°C | Gluconobacter oxydans | |
1.06 | - |
5-(aziridin-1-yl)-2,4-dinitrobenzamide | pH 8.0, 37°C | Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Gluconobacter oxydans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
2-chloronitrobenzene | pH 8.0, 37°C | Gluconobacter oxydans | |
0.33 | - |
nitrobenzene | pH 8.0, 37°C | Gluconobacter oxydans | |
0.39 | - |
4-chloronitrobenzene | pH 8.0, 37°C | Gluconobacter oxydans | |
0.46 | - |
4-nitrotoluene | pH 8.0, 37°C | Gluconobacter oxydans | |
0.7 | - |
3-nitrotoluene | pH 8.0, 37°C | Gluconobacter oxydans | |
1.9 | - |
1,3-dinitrobenzene | pH 8.0, 37°C | Gluconobacter oxydans | |
6.9 | - |
NADPH | pH 8.0, 37°C | Gluconobacter oxydans | |
20 | - |
5-(aziridin-1-yl)-2,4-dinitrobenzamide | pH 8.0, 37°C | Gluconobacter oxydans | |
60 | - |
(2E)-2-[(5-nitrofuran-2-yl)methylidene]hydrazine-1-carboxamide | pH 8.0, 37°C | Gluconobacter oxydans |