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Literature summary for 1.7.1.16 extracted from

  • Berne, C.; Betancor, L.; Luckarift, H.R.; Spain, J.C.
    Application of a microfluidic reactor for screening cancer prodrug activation using silica-immobilized nitrobenzene nitroreductase (2006), Biomacromolecules, 7, 2631-2636.
    View publication on PubMed
Search for more literature for 1.7.1.16

Application

Application Comment Organism
analysis immobilization of enzyme on polyethyleneimine-mediated silica formation, enzyme activity is significantly more stable than NbzA in solution. A microfluidic microreactor is suitable for continuous operation using nitrobenzene, CB1954, and the proantibiotic nitrofurazone. The flow-through system provides a rapid and reproducible screening method for determining the NbzA-catalyzed activation of prodrugs and proantibiotics Pseudomonas oleovorans

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas oleovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 nitrobenzene imobilized enzyme, 25°C, pH not specified in the publication Pseudomonas oleovorans
0.0023
-
 nitrobenzene 25°C, pH not specified in the publication Pseudomonas oleovorans
0.0117
-
 5-(aziridin-1-yl)-2,4-dinitrobenzamide 25°C, pH not specified in the publication Pseudomonas oleovorans
0.0337
-
 5-(aziridin-1-yl)-2,4-dinitrobenzamide imobilized enzyme, 25°C, pH not specified in the publication Pseudomonas oleovorans
1.763
-
 nitrofurazone 25°C, pH not specified in the publication Pseudomonas oleovorans
5.123
-
 nitrofurazone imobilized enzyme, 25°C, pH not specified in the publication Pseudomonas oleovorans

Organism

Organism UniProt Comment Textmining
Pseudomonas oleovorans Q6DLR9
-
-
Pseudomonas oleovorans JS45 Q6DLR9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas oleovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-(aziridin-1-yl)-2,4-dinitrobenzamide + 2 NADPH + 2 H+
-
 Pseudomonas oleovorans ? + 2 NADP+ + H2O enzyme activates the dinitrobenzamide cancer prodrug CB1954, i.e. 5-(aziridin-1-yl)-2,4-dinitrobenzamide ?
5-(aziridin-1-yl)-2,4-dinitrobenzamide + 2 NADPH + 2 H+
-
 Pseudomonas oleovorans JS45 ? + 2 NADP+ + H2O enzyme activates the dinitrobenzamide cancer prodrug CB1954, i.e. 5-(aziridin-1-yl)-2,4-dinitrobenzamide ?
nitrobenzene + 2 NADPH + 2 H+
-
 Pseudomonas oleovorans N-hydroxyaniline + 2 NADP+ + H2O
-
 ?
nitrobenzene + 2 NADPH + 2 H+
-
 Pseudomonas oleovorans JS45 N-hydroxyaniline + 2 NADP+ + H2O
-
 ?
nitrofurazone + 2 NADPH + 2 H+
-
 Pseudomonas oleovorans ? + 2 NADP+ + H2O enzyme activates the proantibiotic nitrofurazone ?
nitrofurazone + 2 NADPH + 2 H+
-
 Pseudomonas oleovorans JS45 ? + 2 NADP+ + H2O enzyme activates the proantibiotic nitrofurazone ?

Synonyms

Synonyms Comment Organism
NbzA
-
 Pseudomonas oleovorans