Cloned (Comment) | Organism |
---|---|
recombinant expression as two separate fragments in Escherichia coli, the N-terminal Nia1-Mo-heme fragment and a C-terminal fragment containing hinge 2 and the FAD-domain, residues 628-917 | Arabidopsis thaliana |
recombinant expression as two separate fragments in Escherichia coli, the N-terminal Nia2-Mo-heme fragment and a C-terminal fragment containing hinge 2 and the FAD-domain, residues 626-917 | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression of the N-terminal fragment of NIA1, residues 1-627, i.e. NIA1-Mo-heme | Arabidopsis thaliana |
additional information | expression of the N-terminal fragment of NIA2, residues 1-625, i.e. NIA2-Mo-heme | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0103 | - |
nitrite | N-terminal plus C-terminal fragment, pH 7, 22°C | Arabidopsis thaliana | |
0.0137 | - |
nitrite | N-terminal fragment, pH 7, 22°C | Arabidopsis thaliana | |
0.0353 | - |
nitrite | N-terminal plus C-terminal fragment, pH 7.5, 22°C | Arabidopsis thaliana | |
0.0355 | - |
nitrite | N-terminal fragment, pH 7.5, 22°C | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P11035 | isoform Nia2, cf. EC 1.7.1.1 | - |
Arabidopsis thaliana | P11832 | isoform Nia1, cf. EC 1.7.1.1 | - |
Renatured (Comment) | Organism |
---|---|
recombinant expression of the N-terminal Nia1-Mo-heme fragment and a C-terminal fragment containing hinge 2 and the FAD-domain, residues 628-917 and mixing of the fragments to restore the electron transfer path | Arabidopsis thaliana |
recombinant expression of the N-terminal Nia2-Mo-heme fragment and a C-terminal fragment containing hinge 2 and the FAD-domain, residues 626-917 and mixing of the fragments to restore the electron transfer path | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + reduced methyl viologen | - |
Arabidopsis thaliana | ammonia + oxidized methyl viologen | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NIA1 | - |
Arabidopsis thaliana |
Nia2 | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
nitrite | N-terminal plus C-terminal fragment, pH 7, 22°C | Arabidopsis thaliana | |
1.8 | - |
nitrite | N-terminal fragment, pH 7, 22°C | Arabidopsis thaliana | |
19.8 | - |
nitrite | N-terminal fragment, pH 7.5, 22°C | Arabidopsis thaliana | |
137.5 | - |
nitrite | N-terminal plus C-terminal fragment, pH 7.5, 22°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
physiological function | isoform NIA1 is the more efficient nitrite reductase while isoform NIA2 exhibits higher nitrate reductase activity | Arabidopsis thaliana |
physiological function | isoform NIA1 is the more efficient nitrite reductase while isoform NIA2 exhibits higher nitrate reductase activity, reaction of EC 1.7.1.1 | Arabidopsis thaliana |