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Literature summary for 1.7.1.15 extracted from

  • Song, Q.; Wang, B.; Zhao, F.; Han, Y.; Zhou, Z.
    Expression, characterization and molecular docking of the assimilatory NADH-nitrite reductase from Acidovorax wautersii QZ-4 (2020), Biochem. Eng. J., 159, 107589 .
No PubMed abstract available
Search for more literature for 1.7.1.15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Acidovorax wautersii

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 87.62% residual activity at 5 mM Acidovorax wautersii
Ca2+ 43.78% residual activity at 5 mM Acidovorax wautersii
Cu2+ 65.3% residual activity at 5 mM Acidovorax wautersii
cyanide 20.75% residual activity at 1 mM Acidovorax wautersii
additional information dithiothreitol and EDTA have no evident effects on enzyme activity Acidovorax wautersii

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+ 131.97% activity at 5 mM Acidovorax wautersii
additional information Mg2+ and Mn2+ have no evident effects on enzyme activity Acidovorax wautersii
Zn2+ 126.21% activity at 5 mM Acidovorax wautersii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrite + 3 NADH + 5 H+ Acidovorax wautersii
-
 NH3 + 3 NAD+ + 2 H2O
-
 ?
nitrite + 3 NADH + 5 H+ Acidovorax wautersii QZ-4
-
 NH3 + 3 NAD+ + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Acidovorax wautersii
-
-
-
Acidovorax wautersii QZ-4
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrite + 3 NADH + 5 H+
-
 Acidovorax wautersii NH3 + 3 NAD+ + 2 H2O
-
 ?
nitrite + 3 NADH + 5 H+
-
 Acidovorax wautersii QZ-4 NH3 + 3 NAD+ + 2 H2O
-
 ?
nitrite + reduced methyl viologen methyl viologen is the best in vitro electron donor Acidovorax wautersii NH3 + oxidized methyl viologen
-
 ?
nitrite + reduced methyl viologen methyl viologen is the best in vitro electron donor Acidovorax wautersii QZ-4 NH3 + oxidized methyl viologen
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 100000 + 1 * 14000, SDS-PAGE Acidovorax wautersii
heterodimer 1 * 91750 + 1 * 12400, calculated from amino acid sequence Acidovorax wautersii

Synonyms

Synonyms Comment Organism
assimilatory NADH-nitrite reductase
-
 Acidovorax wautersii
NirBD
-
 Acidovorax wautersii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 over 93% activity is retained at 40°C. At 20 and 50°C, the enzyme retains more than 73 and 66% activity, respectively Acidovorax wautersii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Acidovorax wautersii

pH Range

pH Minimum pH Maximum Comment Organism
5 7 the enzyme exhibits 95 % of its maximum activity at pH 6.5. At pH 5.0 and 7.0, it remains over 80% relative activity Acidovorax wautersii

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Acidovorax wautersii

pI Value

Organism Comment pI Value Maximum pI Value
Acidovorax wautersii subunit nirD, calculated from amino acid sequence
-
 5.7
Acidovorax wautersii subunit nirB, calculated from amino acid sequence
-
 6.3