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Literature summary for 1.7.1.14 extracted from

  • Watsuji, T.O.; Takaya, N.; Nakamura, A.; Shoun, H.
    A possible role of NADPH-dependent cytochrome P450nor isozyme in glycolysis under denitrifying conditions (2003), Biosci. Biotechnol. Biochem., 67, 1109-1114.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol isoforms P450nor1 and P450nor2 Fusarium lichenicola 5829
-
mitochondrion isoform P450nor1 Fusarium lichenicola 5739
-

Organism

Organism UniProt Comment Textmining
Fusarium lichenicola
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NO + NADH + H+ isozyme P450nor1 uses specifically NADH as electron donor, while isozyme P450Nor2 prefers NADPH to NADH Fusarium lichenicola N2O + NAD+ + H2O
-
?
NO + NADP + H+ isozyme P450Nor2 prefers NADPH to NADH Fusarium lichenicola N2O + NADP+ + H2O
-
?

Synonyms

Synonyms Comment Organism
cytochrome P450nor
-
Fusarium lichenicola
NADPH-dependent cytochrome P450nor
-
Fusarium lichenicola
P450nor1 NADPH-specific isozyme Fusarium lichenicola
P450nor2 isozyme preferring NADPH to NADH Fusarium lichenicola

Cofactor

Cofactor Comment Organism Structure
heme
-
Fusarium lichenicola
NADH isozyme P450nor1 uses specifically NADH as electron donor, while isozyme P450Nor2 prefers NADPH to NADH Fusarium lichenicola
NADPH isozyme P450nor1 uses specifically NADH as electron donor, while isozyme P450Nor2 prefers NADPH to NADH Fusarium lichenicola

pI Value

Organism Comment pI Value Maximum pI Value
Fusarium lichenicola isozyme P450nor1, isoelectric focusing
-
4.4
Fusarium lichenicola isozyme P450nor1, isoelectric focusing
-
5.2

Expression

Organism Comment Expression
Fusarium lichenicola the addition of glucose as a carbon source to the culture medium leads to the production of much more isozyme P450nor2 than a non-fermentable substrate (glycerol or acetate) does up

General Information

General Information Comment Organism
metabolism isozyme P450nor2 acts as the electron sink under anaerobic, denitrifying conditions to regenerate NADP+ for the pentose phosphate cycle Fusarium lichenicola