Cloned (Comment) | Organism |
---|---|
a 404 amino acid fragment of Nor1p is expressed in Escherichia coli JM109 cells | Histoplasma capsulatum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NO | in 100 mM potassium phosphate (pH 7.5), at 25°C | Histoplasma capsulatum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Histoplasma capsulatum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Poros HQ anion-exchange column chromatography | Histoplasma capsulatum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DEA-NONOate + NADH + H+ | - |
Histoplasma capsulatum | N2O + NAD+ + ? | - |
? | |
NO + NADH + H+ | - |
Histoplasma capsulatum | N2O + NAD+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cytochrome P450 nitric oxide reductase | - |
Histoplasma capsulatum |
Nor1p | - |
Histoplasma capsulatum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
83.3 | - |
NO | in 100 mM potassium phosphate (pH 7.5), at 25°C | Histoplasma capsulatum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Histoplasma capsulatum | |
NADH | Nor1p oxidizes NADH but has no activity with NADPH | Histoplasma capsulatum |
General Information | Comment | Organism |
---|---|---|
physiological function | Nor1p plays a protective role against macrophage-derived nitric oxide | Histoplasma capsulatum |