Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109(DE3) cells | Cutaneotrichosporon cutaneum |
Protein Variants | Comment | Organism |
---|---|---|
S73G/S75GS75G | the mutant exhibits 79% activity of wild type with NADH | Fusarium oxysporum |
S75G | the mutant exhibits 63% activity of wild type with NADH | Fusarium oxysporum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Cutaneotrichosporon cutaneum | 5739 | - |
mitochondrion | - |
Aspergillus oryzae | 5739 | - |
mitochondrion | - |
Fusarium oxysporum | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45200 | - |
calculated from amino acid sequence | Cutaneotrichosporon cutaneum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Cutaneotrichosporon cutaneum | - |
- |
- |
Fusarium lichenicola | - |
- |
- |
Fusarium oxysporum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE cellulose column chromatography, phenyl-Superose column chromatography, and Mono Q column chromatography or Resource Q column chromatography | Cutaneotrichosporon cutaneum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | P450nor is a reductase without monooxygenase activity | Cutaneotrichosporon cutaneum | ? | - |
? | |
additional information | P450nor is a reductase without monooxygenase activity | Aspergillus oryzae | ? | - |
? | |
additional information | P450nor is a reductase without monooxygenase activity | Fusarium oxysporum | ? | - |
? | |
additional information | P450nor is a reductase without monooxygenase activity | Fusarium lichenicola | ? | - |
? | |
NO + NADH + H+ | - |
Cutaneotrichosporon cutaneum | N2O + NAD+ + H2O | - |
? | |
NO + NADH + H+ | - |
Aspergillus oryzae | N2O + NAD+ + H2O | - |
? | |
NO + NADH + H+ | - |
Fusarium lichenicola | N2O + NAD+ + H2O | - |
? | |
NO + NADH + H+ | Fnor is specific for NADH | Fusarium oxysporum | N2O + NAD+ + H2O | - |
? | |
NO + NADPH + H+ | - |
Cutaneotrichosporon cutaneum | N2O + NADP+ + H2O | - |
? | |
NO + NADPH + H+ | - |
Aspergillus oryzae | N2O + NADP+ + H2O | - |
? | |
NO + NADPH + H+ | - |
Fusarium lichenicola | N2O + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Anor | - |
Aspergillus oryzae |
Cnor1 | isozyme | Fusarium lichenicola |
Cnor2 | isozyme | Fusarium lichenicola |
CYP55A1 | - |
Fusarium oxysporum |
CYP55A2 | - |
Fusarium lichenicola |
CYP55A3 | - |
Fusarium lichenicola |
CYP55A4 | - |
Cutaneotrichosporon cutaneum |
CYP55A5 | - |
Aspergillus oryzae |
cytochrome P450nor | - |
Cutaneotrichosporon cutaneum |
cytochrome P450nor | - |
Aspergillus oryzae |
cytochrome P450nor | - |
Fusarium oxysporum |
cytochrome P450nor | - |
Fusarium lichenicola |
Fnor | - |
Fusarium oxysporum |
fungal nitric oxide reductase | - |
Cutaneotrichosporon cutaneum |
fungal nitric oxide reductase | - |
Aspergillus oryzae |
fungal nitric oxide reductase | - |
Fusarium oxysporum |
fungal nitric oxide reductase | - |
Fusarium lichenicola |
P450nor | - |
Cutaneotrichosporon cutaneum |
P450nor | - |
Aspergillus oryzae |
P450nor | - |
Fusarium oxysporum |
P450nor | - |
Fusarium lichenicola |
Tnor | - |
Cutaneotrichosporon cutaneum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | heme-thiolate protein | Cutaneotrichosporon cutaneum | |
heme | heme-thiolate protein | Aspergillus oryzae | |
heme | heme-thiolate protein | Fusarium oxysporum | |
heme | heme-thiolate protein | Fusarium lichenicola | |
NADH | Anor can utilize both NADH and NADPH | Aspergillus oryzae | |
NADH | Fnor is specific for NADH | Fusarium oxysporum | |
NADH | isozyme Cnor1 is specific for NADH | Fusarium lichenicola | |
NADH | Tnor can utilize both NADH and NADPH | Cutaneotrichosporon cutaneum | |
NADPH | Anor can utilize both NADH and NADPH | Aspergillus oryzae | |
NADPH | isozyme Cnor2 can utilize both NADH and NADPH | Fusarium lichenicola | |
NADPH | Nor activity of wild type with NADPH as electron donor is only 13% of that with NADH as electron donor | Fusarium oxysporum | |
NADPH | Tnor can utilize both NADH and NADPH | Cutaneotrichosporon cutaneum |
General Information | Comment | Organism |
---|---|---|
physiological function | P450nor is involved in fungal denitrification, it receives electrons directly from the distal pocket from NADH and/or NADPH without any help from a flavoprotein | Cutaneotrichosporon cutaneum |
physiological function | P450nor is involved in fungal denitrification, it receives electrons directly from the distal pocket from NADH and/or NADPH without any help from a flavoprotein | Aspergillus oryzae |
physiological function | P450nor is involved in fungal denitrification, it receives electrons directly from the distal pocket from NADH and/or NADPH without any help from a flavoprotein | Fusarium oxysporum |
physiological function | P450nor is involved in fungal denitrification, it receives electrons directly from the distal pocket from NADH and/or NADPH without any help from a flavoprotein | Fusarium lichenicola |