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Literature summary for 1.7.1.13 extracted from

  • Ribeiro, A.; Yang, L.; Ramos, M.; Fernandes, P.; Liang, Z.; Hirao, H.
    Insight into enzymatic nitrile reduction QM/MM study of the catalytic mechanism of QueF nitrile reductase (2015), ACS Catal., 5, 3740-3751 .
No PubMed abstract available
Search for more literature for 1.7.1.13

Organism

Organism UniProt Comment Textmining
Vibrio cholerae O1 Q9KTK0
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Vibrio cholerae O1 ATCC 39315 Q9KTK0
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-cyano-7-carbaguanine + 2 NADPH + 2 H+
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 Vibrio cholerae O1 7-aminomethyl-7-carbaguanine + 2 NADP+
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 ?
7-cyano-7-carbaguanine + 2 NADPH + 2 H+
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 Vibrio cholerae O1 ATCC 39315 7-aminomethyl-7-carbaguanine + 2 NADP+
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 ?
additional information the nitrile to amine conversion proceeds through four major stages: formation of a C-S covalent bond between the substrate and the catalytic cysteine residue to form the thioimidate intermediate, hydride transfer from NADPH to the substrate to generate the thiohemiaminal intermediate, cleavage of the C-S covalent bond to generate the imine intermediate, and second hydride transfer from NADPH to the imine intermediate to generate the final amine product. The free energy barrier for the rate-limiting step, i.e. the second hydride transfer, is20.8 kcal/mol Vibrio cholerae O1 ?
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 ?
additional information the nitrile to amine conversion proceeds through four major stages: formation of a C-S covalent bond between the substrate and the catalytic cysteine residue to form the thioimidate intermediate, hydride transfer from NADPH to the substrate to generate the thiohemiaminal intermediate, cleavage of the C-S covalent bond to generate the imine intermediate, and second hydride transfer from NADPH to the imine intermediate to generate the final amine product. The free energy barrier for the rate-limiting step, i.e. the second hydride transfer, is20.8 kcal/mol Vibrio cholerae O1 ATCC 39315 ?
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 ?

Synonyms

Synonyms Comment Organism
NADPH-dependent 7-cyano-7-deazaguanine reductase
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 Vibrio cholerae O1
QueF
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 Vibrio cholerae O1

Cofactor

Cofactor Comment Organism Structure
NADPH
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 Vibrio cholerae O1