BRENDA - Enzyme Database show
show all sequences of 1.7.1.13

High-resolution structure of the nitrile reductase QueF combined with molecular simulations provide insight into enzyme mechanism

Kim, Y.; Zhou, M.; Moy, S.; Morales, J.; Cunningham, M.A.; Joachimiak, A.; J. Mol. Biol. 404, 127-137 (2010)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
hanging drop vapor diffusion method, using 0.04 M sodium dihydrogen phosphate, 0.96 M dipotassium hydrogen phosphate, and 10 mM GTP
Vibrio cholerae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
2 * 33000, gel filtration
Vibrio cholerae
67900
-
gel filtration
Vibrio cholerae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Vibrio cholerae
Q9KTK0
-
-
Vibrio cholerae ATCC 39315
Q9KTK0
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA agarose column chromatography
Vibrio cholerae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7-cyano-7-deazaguanine + 2 NADPH + 2 H+
-
712770
Vibrio cholerae
queuine + 2 NADP+
queuine is 7-aminomethyl-7-deazaguanine
-
-
?
7-cyano-7-deazaguanine + 2 NADPH + 2 H+
-
712770
Vibrio cholerae ATCC 39315
queuine + 2 NADP+
queuine is 7-aminomethyl-7-deazaguanine
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 33000, gel filtration
Vibrio cholerae
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Vibrio cholerae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Vibrio cholerae
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop vapor diffusion method, using 0.04 M sodium dihydrogen phosphate, 0.96 M dipotassium hydrogen phosphate, and 10 mM GTP
Vibrio cholerae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
2 * 33000, gel filtration
Vibrio cholerae
67900
-
gel filtration
Vibrio cholerae
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA agarose column chromatography
Vibrio cholerae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7-cyano-7-deazaguanine + 2 NADPH + 2 H+
-
712770
Vibrio cholerae
queuine + 2 NADP+
queuine is 7-aminomethyl-7-deazaguanine
-
-
?
7-cyano-7-deazaguanine + 2 NADPH + 2 H+
-
712770
Vibrio cholerae ATCC 39315
queuine + 2 NADP+
queuine is 7-aminomethyl-7-deazaguanine
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 33000, gel filtration
Vibrio cholerae
Other publictions for EC 1.7.1.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742061
Mohammad
Protection of the queuosine b ...
Bacillus subtilis 168, Bacillus subtilis
Biomolecules
7
E30
2017
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-
1
1
2
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1
-
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2
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1
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1
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742231
Gjonaj
-
Substrate and cofactor bindin ...
Escherichia coli
Catal. Sci. Technol.
6
7391-7397
2016
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-
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1
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1
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1
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1
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2
1
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742900
Jung
Kinetic analysis and probing ...
Escherichia coli
J. Biol. Chem.
291
25411-25426
2016
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1
-
1
3
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2
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3
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2
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1
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1
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1
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1
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3
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3
-
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2
-
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-
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-
-
-
743107
Li
-
Biochemical properties of a n ...
Pectobacterium carotovorum subsp. carotovorum, Pectobacterium carotovorum subsp. carotovorum PCC21
J. Mol. Catal. B
131
47-54
2016
-
-
1
1
-
-
1
2
-
4
1
-
-
2
-
-
-
-
-
-
-
-
4
1
1
1
3
2
1
1
-
1
-
-
-
-
-
1
1
1
-
-
-
1
-
2
-
4
1
-
-
-
-
-
-
-
-
-
4
1
1
1
3
2
1
1
-
-
-
-
-
-
-
-
741484
Ribeiro
-
Insight into enzymatic nitril ...
Vibrio cholerae O1, Vibrio cholerae O1 ATCC 39315
ACS Catal.
5
3740-3751
2015
-
-
-
-
-
-
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-
-
-
-
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2
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4
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1
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1
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-
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4
-
-
-
-
-
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724767
Wilding
Targeting the substrate bindin ...
Escherichia coli
Chemistry
19
7007-7012
2013
-
-
1
1
8
-
-
3
-
-
-
1
-
2
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10
1
3
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3
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1
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1
8
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3
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1
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10
1
3
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3
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724879
Moeller
Expression and characterizatio ...
Escherichia coli
Enzyme Microb. Technol.
52
129-133
2013
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2
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2
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2
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1
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3
2
1
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2
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2
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2
-
1
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3
2
1
-
2
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-
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712770
Kim
High-resolution structure of t ...
Vibrio cholerae, Vibrio cholerae ATCC 39315
J. Mol. Biol.
404
127-137
2010
-
-
-
1
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-
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-
-
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2
-
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3
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1
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2
1
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1
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1
1
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2
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1
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2
1
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660634
Swairjo
Crystallization and preliminar ...
Bacillus subtilis
Acta Crystallogr. Sect. F
F61
945-948
2005
-
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1
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1
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2
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1
1
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1
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1
1
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1
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1
1
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663244
van Lanen
From cyclohydrolase to oxidore ...
Bacillus subtilis, Escherichia coli
Proc. Natl. Acad. Sci. USA
102
4264-4269
2005
-
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2
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1
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2
-
7
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2
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2
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1
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2
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2
2
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1
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2
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2
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2
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1
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662983
Okada
Structure determination of a n ...
Escherichia coli
Nucleic Acids Res.
5
2289-2296
1978
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1
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1
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662984
Noguchi
Isolation of Q nucleoside prec ...
Escherichia coli
Nucleic Acids Res.
5
4215-4223
1978
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1
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1
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662966
Kuchino
Biosynthesis of the modified n ...
Salmonella enterica subsp. enterica serovar Typhimurium
Nucleic Acids Res.
3
393-398
1976
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