Literature summary for 1.7.1.13 extracted from

van Lanen, S.G.; Reader, J.S.; Swairjo, M.A.; de Crecy-Lagard, V.; Lee, B.; Iwata-Reuyl, D.
From cyclohydrolase to oxidoreductase: discovery of nitrile reductase activity in a common fold (2005), Proc. Natl. Acad. Sci. USA, 102, 4264-4269.

Search for more literature for 1.7.1.13

Cloned(Commentary)

Cloned (Comment)	Organism
overproduction in Escherichia coli	Bacillus subtilis
overproduction in Escherichia coli	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.036	-	NADPH	-	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
7-cyano-7-deazaguanine + 2 NADPH + 2 H+	Bacillus subtilis	late step in biosynthesis of the modified tRNA nucleoside queuosine	queuine + 2 NADP+	-	?
7-cyano-7-deazaguanine + 2 NADPH + 2 H+	Escherichia coli	late step in biosynthesis of the modified tRNA nucleoside queuosine	queuine + 2 NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	-	-	-
Escherichia coli	-	BL21 (DE3)	-

Purification (Commentary)

Purification (Comment)	Organism
-	Bacillus subtilis
-	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
7-cyano-7-deazaguanine + 2 NADPH + 2 H+	late step in biosynthesis of the modified tRNA nucleoside queuosine	Bacillus subtilis	queuine + 2 NADP+	-	?
7-cyano-7-deazaguanine + 2 NADPH + 2 H+	late step in biosynthesis of the modified tRNA nucleoside queuosine	Escherichia coli	queuine + 2 NADP+	-	?

Synonyms

Synonyms	Comment	Organism
QueF	-	Bacillus subtilis
QueF	-	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.01	-	NADPH	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Bacillus subtilis
NADPH	-	Escherichia coli

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Release 2023.1 (January 2023)