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Literature summary for 1.7.1.10 extracted from

  • Cabello, P.; Pino, C.; Olmo-Mira, M.F.; Castillo, F.; Roldan, M.D.; Moreno-Vivian, C.
    Hydroxylamine assimilation by Rhodobacter capsulatus E1F1. Requirement of the hcp gene (hybrid cluster protein) located in the nitrate assimilation nas gene region for hydroxylamine reduction (2004), J. Biol. Chem., 279, 45485-45494.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 as His-tag fusion protein Rhodobacter capsulatus

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl
-
Rhodobacter capsulatus
CN-
-
Rhodobacter capsulatus
dithioerythritol
-
Rhodobacter capsulatus
o-phenanthroline strong inhibition Rhodobacter capsulatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
methyl viologen pH 9.3, 40°C Rhodobacter capsulatus
1
-
hydroxylamine pH 9.3, 40°C Rhodobacter capsulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
SDS-PAGE Rhodobacter capsulatus
57800
-
calculated from the deduced amino acid sequence Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hydroxylamine + NADH Rhodobacter capsulatus involved in the assimilation of toxic hydroxylamine formed during nitrate assimilation NH3 + NAD+ + H2O
-
?
hydroxylamine + NADH Rhodobacter capsulatus E1F1 involved in the assimilation of toxic hydroxylamine formed during nitrate assimilation NH3 + NAD+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Rhodobacter capsulatus
-
highest activity in cells grown in the presence of hydroxylamine
-
Rhodobacter capsulatus E1F1
-
highest activity in cells grown in the presence of hydroxylamine
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein using His-tag Rhodobacter capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hydroxylamine + methyl viologen
-
Rhodobacter capsulatus NH3 + H2O + ?
-
?
hydroxylamine + methyl viologen
-
Rhodobacter capsulatus E1F1 NH3 + H2O + ?
-
?
hydroxylamine + NADH involved in the assimilation of toxic hydroxylamine formed during nitrate assimilation Rhodobacter capsulatus NH3 + NAD+ + H2O
-
?
hydroxylamine + NADH involved in the assimilation of toxic hydroxylamine formed during nitrate assimilation Rhodobacter capsulatus E1F1 NH3 + NAD+ + H2O
-
?
hydroxylamine + NADH only native, not recombinant protein, recombinant protein lacks NADH oxidoreductase activity Rhodobacter capsulatus NH3 + H2O + NAD+
-
?
hydroxylamine + NADH only native, not recombinant protein, recombinant protein lacks NADH oxidoreductase activity Rhodobacter capsulatus E1F1 NH3 + H2O + NAD+
-
?

Synonyms

Synonyms Comment Organism
hybrid cluster protein formerly primane protein, displays hydroxylamine reductase activity Rhodobacter capsulatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Rhodobacter capsulatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.3
-
-
Rhodobacter capsulatus

Cofactor

Cofactor Comment Organism Structure
iron-sulfur centre [2Fe-2S] and [4Fe-2S-2O] Rhodobacter capsulatus
NADH
-
Rhodobacter capsulatus