Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 as His-tag fusion protein | Rhodobacter capsulatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-dipyridyl | - |
Rhodobacter capsulatus | |
CN- | - |
Rhodobacter capsulatus | |
dithioerythritol | - |
Rhodobacter capsulatus | |
o-phenanthroline | strong inhibition | Rhodobacter capsulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
methyl viologen | pH 9.3, 40°C | Rhodobacter capsulatus | |
1 | - |
hydroxylamine | pH 9.3, 40°C | Rhodobacter capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
SDS-PAGE | Rhodobacter capsulatus |
57800 | - |
calculated from the deduced amino acid sequence | Rhodobacter capsulatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxylamine + NADH | Rhodobacter capsulatus | involved in the assimilation of toxic hydroxylamine formed during nitrate assimilation | NH3 + NAD+ + H2O | - |
? | |
hydroxylamine + NADH | Rhodobacter capsulatus E1F1 | involved in the assimilation of toxic hydroxylamine formed during nitrate assimilation | NH3 + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | - |
highest activity in cells grown in the presence of hydroxylamine | - |
Rhodobacter capsulatus E1F1 | - |
highest activity in cells grown in the presence of hydroxylamine | - |
Purification (Comment) | Organism |
---|---|
recombinant protein using His-tag | Rhodobacter capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxylamine + methyl viologen | - |
Rhodobacter capsulatus | NH3 + H2O + ? | - |
? | |
hydroxylamine + methyl viologen | - |
Rhodobacter capsulatus E1F1 | NH3 + H2O + ? | - |
? | |
hydroxylamine + NADH | involved in the assimilation of toxic hydroxylamine formed during nitrate assimilation | Rhodobacter capsulatus | NH3 + NAD+ + H2O | - |
? | |
hydroxylamine + NADH | involved in the assimilation of toxic hydroxylamine formed during nitrate assimilation | Rhodobacter capsulatus E1F1 | NH3 + NAD+ + H2O | - |
? | |
hydroxylamine + NADH | only native, not recombinant protein, recombinant protein lacks NADH oxidoreductase activity | Rhodobacter capsulatus | NH3 + H2O + NAD+ | - |
? | |
hydroxylamine + NADH | only native, not recombinant protein, recombinant protein lacks NADH oxidoreductase activity | Rhodobacter capsulatus E1F1 | NH3 + H2O + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hybrid cluster protein | formerly primane protein, displays hydroxylamine reductase activity | Rhodobacter capsulatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Rhodobacter capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.3 | - |
- |
Rhodobacter capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
iron-sulfur centre | [2Fe-2S] and [4Fe-2S-2O] | Rhodobacter capsulatus | |
NADH | - |
Rhodobacter capsulatus |