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Literature summary for 1.7.1.10 extracted from
- The hydroxylamine reductase of mitochondria (1969), Arch. Biochem. Biophys., 134, 408-413.
General Stability
| General Stability | Organism |
|---|---|
| dithiothreitol stabilizes during storage | Rattus norvegicus |
| freezing partially inactivates membrane bound enzyme | Rattus norvegicus |
| NADH prevents heat damage | Rattus norvegicus |
| solubilization with deoxycholate, n-butanol or acetone destroys membrane bound enzyme | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| HgCl2 | - |
Rattus norvegicus |  |
| N-ethylmaleimide | not inhibited | Rattus norvegicus | |
| p-chloromercuribenzoate | 0.05 mM, complete inhibition, subsequent addition of 3 mM cysteine restores the activity | Rattus norvegicus | |
| phosphate | - |
Rattus norvegicus | |
| Sodium arsenate | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
hydroxylamine | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | firmly attached to mitochondrial membrane | Rattus norvegicus | 16020 | - |
| mitochondrion | firmly attached to membrane | Rattus norvegicus | 5739 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydroxylamine + NADH | possible identity of hydroxylamine reductase and the enzyme which reduces anthranilic hydroxamic acid to the amide | Rattus norvegicus | NH3 + NAD+ + H2O | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
NADH prevents heat damage | Rattus norvegicus |
| 40 | - |
membrane bound enzyme, 10 min, pH 7.4, dithiothreitol, no loss of activity | Rattus norvegicus |
| 50 | - |
membrane bound enzyme, 10 min, pH 7.4, dithiothreitol, 50% loss of activity | Rattus norvegicus |
| 60 | - |
membrane bound enzyme, pH 7.4, dithiothreitol, complete loss of activity | Rattus norvegicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | - |
complete loss of activity after brief exposure | Rattus norvegicus |
| 8.4 | - |
no loss of activity after brief exposure | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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