Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-cyclohexyl-3-(2-morpholinoethyl)carbodiimide metho-p-toluenesulfonate | 1.5 mM, 68% inhibition, hydroxylamine protects | Derxia gummosa | |
2,2'-dipyridyl | - |
Derxia gummosa | |
8-hydroxyquinoline | 0.1 mM, 50% inhibition of hydroxylamine reductase activity | Derxia gummosa | |
Antimycin 1 | 0.1 mM, 26% inhibition of hydroxylamine reductase activity | Derxia gummosa | |
dinitrophenol | 0.1 mM, 80% inhibition of hydroxylamine reductase activity | Derxia gummosa | |
Iodine | 0.5 mM, 80% inhibition, preincubation with hydroxylamine protects | Derxia gummosa | |
N-ethylmaleimide | 0.1 mM, 58% inhibition of hydroxylamine reductase activity | Derxia gummosa | |
NADH | inhibits in absence of hydroxylamine | Derxia gummosa | |
nitrite | nitrite and hydroxylamine reductase activity are associated with the same enzyme, nitrite and hydroxylamine are competitive for the same binding site | Derxia gummosa | |
p-chloromercuribenzoate | 0.1 mM, 67% inhibition of hydroxylamine reductase activity | Derxia gummosa | |
potassium cyanide | - |
Derxia gummosa | |
potassium thiocyanate | 0.1 mM, 90% inhibition of hydroxylamine reductase activity, nitrite reductase activity is not inhibited | Derxia gummosa | |
Rose bengal | 0.5 mM, 94% inhibition | Derxia gummosa | |
rotenone | 0.1 mM, 52% inhibition of hydroxylamine reductase activity | Derxia gummosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0048 | - |
NO2- | - |
Derxia gummosa | |
0.15 | - |
NADH | - |
Derxia gummosa | |
5.3 | - |
hydroxylamine | - |
Derxia gummosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Derxia gummosa | - |
nitrite and hydroxylamine reductase are associated within the same enzyme | - |
Purification (Comment) | Organism |
---|---|
nitrite and hydroxylamine reductase are associated with the same enzyme | Derxia gummosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.422 | - |
reduction of nitrite | Derxia gummosa |
0.68 | - |
reduction of hydroxylamine | Derxia gummosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxylamine + NADH | 100% activity with NADH, 46% with NADPH, 117% with FADH2, 105% with FMNH2, 220% with methylviologen, 210% with benzyl viologen, 57% with Na2S2O4 | Derxia gummosa | NH3 + NAD+ + H2O | - |
? | |
NO2- + NADH | enzyme has both nitrite reductase and hydroxylamine reductase activity, main function may be nitrite reduction | Derxia gummosa | NH3 + H2O + NAD+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Derxia gummosa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
NO2- | - |
Derxia gummosa |