Activating Compound | Comment | Organism | Structure |
---|---|---|---|
8-bromo-cyclic GMP | addition of 8Br-cGMP, an analogous compound of the secondary messenger cGMP and a secondary signal molecule itself, increases significantly nitrate reductase activity by 51% at 0.01 mM | Agarophyton chilense | |
methylene blue | a guanyl cyclase inhibitor, increases significantly the enzymatic activity by 141% at 0.05 mM | Agarophyton chilense |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone | a photosynthetic electron flux inhibitor of photosystem I, causes 61% inhibition at 0.008 mM | Agarophyton chilense | |
2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide | a NO scavenger | Agarophyton chilense | |
3-(3,4-dichlorophenyl)-1,1-dimethylurea | a photosynthetic electron flux inhibitor of photosystem II, causes 51% inhibition at 0.001 mM | Agarophyton chilense | |
additional information | no enzyme inhibition by diphenyleneiodonium, i.e. DPI, a NADPH oxidase inhibitor, and poor inhibition by L-NG-monomethyl arginine citrate (L-NMMA), a NOS inhibitor. But nitrate reductase activity is sensitive to a decrease or increase of NO levels when NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide and NO donor sodium nitroprusside are added | Agarophyton chilense | |
sodium nitroprusside | a NO donor | Agarophyton chilense |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Agarophyton chilense | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Molybdenum | - |
Agarophyton chilense |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + NADH + H+ | Agarophyton chilense | - |
nitrite + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agarophyton chilense | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + NADH + H+ | - |
Agarophyton chilense | nitrite + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Agarophyton chilense |
Synonyms | Comment | Organism |
---|---|---|
nitrate reductase | - |
Agarophyton chilense |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Agarophyton chilense |
General Information | Comment | Organism |
---|---|---|
metabolism | nitric oxide is an important signaling molecule regulating nitrate reductase activity, and cGMP participates as secondary messenger on this regulation by phosphorylation and desphosphorylation processes | Agarophyton chilense |
physiological function | nitrate reductase is the first enzyme in the nitrogen assimilatory pathway, which reduces nitrate to nitrite. Nitrate reductase is a key enzyme in nitrogen metabolism, that has been implicated in the production of nitric oxide (NO) in plants. In the red macroalga, nitrate reductase activity is modulated by photosynthetic electron transport chain and nitric oxide balance, direct dependence of nitrate reductase activity on the PSII and PSI electron flux, overview | Agarophyton chilense |