Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.7.1.1 extracted from

  • Iwamoto, K.; Shiraiwa, Y.
    Characterization of NADH: nitrate reductase from the coccolithophorid Emiliania huxleyi (Lohman) Hay & Mohler (Haptophyceae) (2003), Mar. Biotechnol., 5, 20-26.
    View publication on PubMed
Search for more literature for 1.7.1.1

Inhibitors

Inhibitors Comment Organism Structure
KCN 0.1 mM, complete inhibition Emiliania huxleyi
N-ethylmaleimide 1 mM, complete inhibition Emiliania huxleyi
NaN3 0.1 mM, complete inhibition Emiliania huxleyi
o-phenanthroline 1 mM, complete inhibition Emiliania huxleyi
p-chloromercuribenzoic acid 0.01 mM, complete inhibition Emiliania huxleyi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 NADH pH 8.5, 25°C Emiliania huxleyi
0.104
-
 nitrate pH 8.5, 25°C Emiliania huxleyi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
 6 * 85000, SDS-PAGE Emiliania huxleyi
514000
-
 gel filtration Emiliania huxleyi

Organism

Organism UniProt Comment Textmining
Emiliania huxleyi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Emiliania huxleyi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
52.33
-
 pH 8.5, 25°C Emiliania huxleyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + nitrate + H+
-
 Emiliania huxleyi NAD+ + H2O + nitrite
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 85000, SDS-PAGE Emiliania huxleyi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Emiliania huxleyi