Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
C191A | enzyme is still produced, but it is inactive | Arabidopsis thaliana |
C191S | enzyme is still produced, but it is inactive | Arabidopsis thaliana |
S266A | 48% of the activity of wild-type enzyme | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
NADH | wild-type recombinant enzyme | Arabidopsis thaliana | |
0.015 | - |
nitrate | wild-type recombinant enzyme | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme expressed in Pichia pastoris | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
32 | - |
- |
Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + NADH | - |
Arabidopsis thaliana | nitrite + NAD+ + H2O | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
wild-type recombinant enzyme | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Arabidopsis thaliana |