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Literature summary for 1.7.1.1 extracted from

  • Su, W.; Mertens, J.A.; Kanamaru, K.; Campbell, W.H.; Crawford, N.M.
    Analysis of wild-type and mutant plant nitrate reductase expressed in the methylotrophic yeast Pichia pastoris (1997), Plant Physiol., 115, 1135-1143.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
C191A enzyme is still produced, but it is inactive Arabidopsis thaliana
C191S enzyme is still produced, but it is inactive Arabidopsis thaliana
S266A 48% of the activity of wild-type enzyme Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
NADH wild-type recombinant enzyme Arabidopsis thaliana
0.015
-
nitrate wild-type recombinant enzyme Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme expressed in Pichia pastoris Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
32
-
-
Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrate + NADH
-
Arabidopsis thaliana nitrite + NAD+ + H2O
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
wild-type recombinant enzyme Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADH
-
Arabidopsis thaliana