Protein Variants | Comment | Organism |
---|---|---|
C17S | visible and CD spectra are very similar to that of the wild-type domain, thermal stability is slightly decreased compared to the wild-type domain | Spinacia oleracea |
C240A | decrease of diaphorase activity | Spinacia oleracea |
C240G | decrease of diaphorase activity | Spinacia oleracea |
C240S | decrease of diaphorase activity, thermal stability is slightly decreased compared to the wild-type domain, the oxidation reduction midpoint potential for the FAD/FADH2 couple is -219 mV compared to -268 mV in the wild-type domain | Spinacia oleracea |
C54S | visible and CD spectra are very similar to that of the wild-type domain, thermal stability is slightly decreased compared to the wild-type domain,the oxidation reduction midpoint potential for the FAD/FADH2 couple is -197 mV compared to -268 mV in the wild-type domain | Spinacia oleracea |
C62S | visible and CD spectra are very similar to that of the wild-type domain, thermal stability is decreased compared to the wild-type domain, the oxidation reduction midpoint potential for the FAD/FADH2 couple is -226 mV compared to -268 mV in the wild-type domain | Spinacia oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-(4'-maleimidylanilino)naphthalene-6-sulfonic acid | - |
Chlorella sp. | |
2-(4'-maleimidylanilino)naphthalene-6-sulfonic acid | - |
Spinacia oleracea | |
methyl methanethiosulfonate | - |
Chlorella sp. | |
methyl methanethiosulfonate | inactivation is concentration independent | Spinacia oleracea | |
NAD+ | - |
Spinacia oleracea | |
NEM | - |
Chlorella sp. | |
NEM | - |
Spinacia oleracea | |
PCMB | - |
Chlorella sp. | |
PCMB | inactivation is concentration independent | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
NADH | recombinant flavin domain of nitrate reductase C240A mutant | Spinacia oleracea | |
0.011 | - |
NADH | recombinant flavin domain of nitrate reductase C240G mutant | Spinacia oleracea | |
0.012 | - |
NADH | wild-type recombinant flavin domain of nitrate reductase | Spinacia oleracea | |
0.014 | - |
NADH | recombinant flavin domain of nitrate reductase C240S mutant | Spinacia oleracea | |
0.015 | - |
NADH | recombinant flavin domain of nitrate reductase C17S mutant | Spinacia oleracea | |
0.015 | - |
NADH | recombinant flavin domain of nitrate reductase C54S mutant | Spinacia oleracea | |
0.016 | - |
NADH | recombinant flavin domain of nitrate reductase C62S mutant | Spinacia oleracea | |
0.024 | - |
NADH | recombinant flavin domain of nitrate reductase C240S mutant | Spinacia oleracea | |
0.027 | - |
ferricyanide | wild-type recombinant flavin domain of nitrate reductase | Spinacia oleracea | |
0.036 | - |
ferricyanide | recombinant flavin domain of nitrate reductase C54S mutant | Spinacia oleracea | |
0.045 | - |
ferricyanide | recombinant flavin domain of nitrate reductase C17S mutant | Spinacia oleracea | |
0.059 | - |
NADH | recombinant flavin domain of nitrate reductase C62S mutant | Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorella sp. | - |
- |
- |
Spinacia oleracea | - |
recombinant flavin domain | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricyanide + NADH | - |
Spinacia oleracea | 2 ferrocyanide + NAD+ + H+ | - |
? | |
2 ferricyanide + NADH | - |
Chlorella sp. | 2 ferrocyanide + NAD+ + H+ | - |
? | |
nitrate + NADH | - |
Spinacia oleracea | nitrite + NAD+ + H2O | - |
? | |
nitrate + NADH | - |
Chlorella sp. | nitrite + NAD+ + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Spinacia oleracea | |
NADH | - |
Chlorella sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
NAD+ | recombinant flavin domain of nitrate reductase C240G mutant | Spinacia oleracea | |
0.8 | - |
NAD+ | recombinant flavin domain of nitrate reductase C240A mutant | Spinacia oleracea | |
1.2 | - |
NAD+ | recombinant flavin domain of nitrate reductase C240S mutant | Spinacia oleracea | |
1.5 | - |
NAD+ | recombinant flavin domain of nitrate reductase C54S mutant | Spinacia oleracea | |
1.9 | - |
NAD+ | recombinant flavin domain of nitrate reductase wild-type and C17S mutant | Spinacia oleracea | |
2 | - |
NAD+ | recombinant flavin domain of nitrate reductase C62S mutant | Spinacia oleracea |