General Stability | Organism |
---|---|
the enzyme is stable at low protein concentrations and in the presence of both substrates | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,6-dichlorophenolindophenol | substrate inhibition | Saccharomyces cerevisiae | |
AMP | dead end reversible inhibitor, competitive inhibitor of NADH and uncompetitive inhibitor of 2,6-dichlorophenolindophenol | Saccharomyces cerevisiae | |
flavone | partial inhibitor | Saccharomyces cerevisiae | |
additional information | not inhibited by rotenone, NAD+ does not inhibit the activity of the enzyme, even at concentrations as high as 10 mM | Saccharomyces cerevisiae | |
NADH | substrate inhibition | Saccharomyces cerevisiae | |
reduced 2,6-dichlorophenolindophenol | product inhibition | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0062 | - |
2,6-dichlorophenolindophenol | pH 7.0, 25°C | Saccharomyces cerevisiae | |
0.0094 | - |
NADH | pH 7.0, 25°C | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
- |
Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H+ + ubiquinone | Saccharomyces cerevisiae | - |
NAD+ + ubiquinol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE Bio-gel column chromatography and Blue Sepharose CL-6B column chromatography, column chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the yeast enzyme is a two-electron reaction enzyme | Saccharomyces cerevisiae | ? | - |
? | |
NADH + H+ + 2,6-dichlorophenolindophenol | - |
Saccharomyces cerevisiae | NAD+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
NADH + H+ + ubiquinone | - |
Saccharomyces cerevisiae | NAD+ + ubiquinol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Internal NADH dehydrogenase | - |
Saccharomyces cerevisiae |
NADH: ubiquinone oxidoreductase | - |
Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Saccharomyces cerevisiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | the free enzyme and the binary complex E-NADH are highly stable in the pH range from 5.5 to 8.0, maintaining the initial activity after 10 min incubation. Maximal stability is observed at pH 5.0 and 6.5 with no loss in activity. The enzyme is highly unstable above and below this pH range | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the mature enzyme contains FAD as prosthetic group | Saccharomyces cerevisiae | |
additional information | the mature enzyme contains no iron-sulfur clusters | Saccharomyces cerevisiae | |
NADH | - |
Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0054 | - |
flavone | hyperbolic uncompetitive inhibition of NADH, pH 7.0, 25°C | Saccharomyces cerevisiae | |
0.0071 | - |
flavone | hyperbolic noncompetitive inhibition of 2,6-dichlorophenolindophenol, pH 7.0, 25°C | Saccharomyces cerevisiae | |
0.0141 | - |
reduced 2,6-dichlorophenolindophenol | pH 7.0, 25°C | Saccharomyces cerevisiae | |
0.0525 | - |
NADH | pH 7.0, 25°C | Saccharomyces cerevisiae | |
0.1152 | - |
2,6-dichlorophenolindophenol | pH 7.0, 25°C | Saccharomyces cerevisiae | |
5.5 | - |
AMP | linear competitive inhibition of NADH, pH 7.0, 25°C | Saccharomyces cerevisiae | |
11.5 | - |
AMP | linear uncompetitive inhibition of 2,6-dichlorophenolindophenol, pH 7.0, 25°C | Saccharomyces cerevisiae |