Cloned (Comment) | Organism |
---|---|
gene arsH | Synechocystis sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
menadione | - |
Synechocystis sp. | |
NADP+ | can act as a competitive inhibitor for NADPH binding at the active site of an enzyme | Synechocystis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | stopped-flow and laser-flash photolysis kinetic analyses, steady-state kinetics | Synechocystis sp. | |
0.0012 | - |
dibromothymoquinone | pH 7.5, 10°C | Synechocystis sp. | |
0.0013 | - |
2,5-dimethyl-4-benzoquinone | pH 7.5, 10°C | Synechocystis sp. | |
0.0028 | - |
duroquinone | pH 7.5, 10°C | Synechocystis sp. | |
0.0057 | - |
menadione | pH 7.5, 10°C | Synechocystis sp. | |
0.0119 | - |
coenzyme Q10 | pH 7.5, 10°C | Synechocystis sp. | |
0.0237 | - |
2-Hydroxy-1,4-naphthoquinone | pH 7.5, 10°C | Synechocystis sp. | |
0.0315 | - |
NADPH | pH 7.5, 10°C | Synechocystis sp. | |
0.0345 | - |
anthraquinone-2-sulfonate | pH 7.5, 10°C | Synechocystis sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25900 | - |
4 * 25900, SDS-PAGE | Synechocystis sp. |
108000 | - |
gel filtration | Synechocystis sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + H+ + 2 quinone | Synechocystis sp. | - |
NADP+ + 2 semiquinone | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
gene arsh, encoded in the arsenic resistance operon | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
NADPH + H+ + 2 quinone = NADP+ + 2 semiquinone | ping pong reaction mechanism | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | although the enzyme is able to stabilize the anionic semiquinone form of the FMN, reduction of quinones involves the hydroquinone form of the flavin cofactor, and the enzymatic reaction occurs through a ping pong-type mechanism. ArsH is able to catalyze one-electron reactions (oxygen and cytocrome c reduction), involving the FMN semiquinone form, but with lower efficiency | Synechocystis sp. | ? | - |
? | |
NADPH + H+ + 2 2,5-dimethyl-4-benzoquinone | - |
Synechocystis sp. | NADP+ + 2 2,5-dimethyl-4-benzosemiquinone | - |
? | |
NADPH + H+ + 2 2-hydroxy-1,4-naphthoquinone | - |
Synechocystis sp. | NADP+ + 2 2-hydroxy-1,4-naphthosemiquinone | - |
? | |
NADPH + H+ + 2 anthraquinone-2-sulfonate | - |
Synechocystis sp. | NADP+ + ? | - |
? | |
NADPH + H+ + 2 coenzyme Q10 | - |
Synechocystis sp. | NADP+ + ? | - |
? | |
NADPH + H+ + 2 dibromothymoquinone | - |
Synechocystis sp. | NADP+ + 2 dibromothymosemiquinone | - |
? | |
NADPH + H+ + 2 duroquinone | - |
Synechocystis sp. | NADP+ + 2 durosemiquinone | - |
? | |
NADPH + H+ + 2 menadione | - |
Synechocystis sp. | NADP+ + ? | - |
? | |
NADPH + H+ + 2 quinone | - |
Synechocystis sp. | NADP+ + 2 semiquinone | - |
? | |
NADPH + H+ + 2 quinone | the catalytic cycle of ArsH consists of the acceptance of two electrons from NADPH to reduce the flavin cofactor (reductive half-reaction) and the transfer of these electrons to an acceptor (oxidative half-reaction) | Synechocystis sp. | NADP+ + 2 semiquinone | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 25900, SDS-PAGE | Synechocystis sp. |
Synonyms | Comment | Organism |
---|---|---|
ArsH | - |
Synechocystis sp. |
NADPH-dependent quinone reductase | - |
Synechocystis sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 25 | assay at | Synechocystis sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
coenzyme Q10 | pH 7.5, 10°C | Synechocystis sp. | |
9.4 | - |
anthraquinone-2-sulfonate | pH 7.5, 10°C | Synechocystis sp. | |
11 | - |
2-Hydroxy-1,4-naphthoquinone | pH 7.5, 10°C | Synechocystis sp. | |
25 | - |
duroquinone | pH 7.5, 10°C | Synechocystis sp. | |
26 | - |
menadione | pH 7.5, 10°C | Synechocystis sp. | |
35 | - |
dibromothymoquinone | pH 7.5, 10°C | Synechocystis sp. | |
36.7 | - |
2,5-dimethyl-4-benzoquinone | pH 7.5, 10°C | Synechocystis sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | assay at | Synechocystis sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | in silico structural model of ArsH reconstituted with FMN, overview | Synechocystis sp. |
General Information | Comment | Organism |
---|---|---|
additional information | in silico structural model of ArsH reconstituted with FMN, overview | Synechocystis sp. |
physiological function | ArsH plays a role in the response to oxidative stress caused by arsenite | Synechocystis sp. |