Cloned (Comment) | Organism |
---|---|
expression of His-tagged enzyme in Escherichia coli | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
the quinone oxidoreductase activity of purified his-tagged recombinant mouse zeta-crystallin is comparable to that of purified native guinea pig lens zeta-crystallin, and to that of recombinant guinea pig zeta-crystallin. The method permits production of substantial amounts of recombinant zeta-crystallin for conducting studies on the biological role of this protein | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-hydroxy-1,4-naphthoquinone + NADPH + H+ | i.e. juglone | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
zeta-Crystallin | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Mus musculus |