Literature summary for 1.6.5.5 extracted from

Shimomura, Y.; Kakuta, Y.; Fukuyama, K.
Crystal structures of the quinone oxidoreductase from Thermus thermophilus HB8 and its complex with NADPH: Implication for NADPH and substrate recognition (2003), J. Bacteriol., 185, 4211-4218.

Crystallization (Commentary)

Crystallization (Comment)	Organism
native enzyme and its complex with NADPH at 2.3 A and 2.8 A resolution. QOR forms a homodimer in the crystal by interaction of the betaF-strands in domain II, forming a large beta-sheet that crosses the dimer interface. NADPH is located between the two domains in the QOR-NADPH complex. The disordered segment involved in the coenzyme binding of apo-QOR becomes ordered upon NADPH binding. The segment covers an NADPH-binding cleft and may serve as a lid. The 2'-phosphate group of the adenine of NADPH is surrounded by polar and positively charged residues in QOR, suggesting that QOR binds NADPH more readily than NADH. The putative substrate-binding site of QOR, is largely blocked by nearby residues	Thermus thermophilus

Crystallization (Comment)

Organism

native enzyme and its complex with NADPH at 2.3 A and 2.8 A resolution. QOR forms a homodimer in the crystal by interaction of the betaF-strands in domain II, forming a large beta-sheet that crosses the dimer interface. NADPH is located between the two domains in the QOR-NADPH complex. The disordered segment involved in the coenzyme binding of apo-QOR becomes ordered upon NADPH binding. The segment covers an NADPH-binding cleft and may serve as a lid. The 2'-phosphate group of the adenine of NADPH is surrounded by polar and positively charged residues in QOR, suggesting that QOR binds NADPH more readily than NADH. The putative substrate-binding site of QOR, is largely blocked by nearby residues

Thermus thermophilus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
soluble	-	Thermus thermophilus	-	-

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	Q8L3C8	-	-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579	Q8L3C8	-	-

Synonyms

Synonyms	Comment	Organism
Qor	-	Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
80	-	thermal denaturation	Thermus thermophilus

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	NADPH is located in the cleft between domains I and II. The adenine ring is sandwiched between the main chains of Ala220 and Glu221 and the side chain of Arg292. Ala220 and Glu221 are disordered in apo-QOR	Thermus thermophilus