Literature summary for 1.6.5.4 extracted from

Igamberdiev, A.U.; Bykova, N.V.; Hill, R.D.
Nitric oxide scavenging by barley hemoglobin is facilitated by a monodehydroascorbate reductase-mediated ascorbate reduction of methemoglobin (2006), Planta, 223, 1033-1040.

Search for more literature for 1.6.5.4

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Hordeum vulgare

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.004	-	NADH	in the presence of hemoglobin	Hordeum vulgare
0.6	-	monodehydroascorbate	in the presence of hemoglobin	Hordeum vulgare

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Hordeum vulgare	5829	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	SDS-PAGE	Hordeum vulgare

Organism

Organism	UniProt	Comment	Textmining
Hordeum vulgare	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Phenyl Superose gel filtration, Blue Sepharose column chromatography and DEAE cellulose column chromatography	Hordeum vulgare

Source Tissue

Source Tissue	Comment	Organism	Textmining
root	-	Hordeum vulgare	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
185	-	using ascorbate free radical as substrate	Hordeum vulgare

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NADH + monodehydroascorbate	-	Hordeum vulgare	NAD+ + ascorbate	-	r

Subunits

Subunits	Comment	Organism
monomer	1 * 40000, SDS-PAGE	Hordeum vulgare

Synonyms

Synonyms	Comment	Organism
MDHAR	-	Hordeum vulgare

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Release 2023.1 (January 2023)