Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Spinacia oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dicoumarol | - |
Spinacia oleracea | |
iodoacetate | 5 mM, 58% inhibition | Spinacia oleracea | |
additional information | not inhibited by 5 mM N-ethylmaleimide | Spinacia oleracea | |
p-chloromercuribenzoate | 0.1 mM, 90% inhibition | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0063 | - |
NADH | at 0.003 mM monodehydroascorbate | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Spinacia oleracea | 9507 | - |
cytosol | - |
Spinacia oleracea | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
gel filtration, chloroplastic enzyme | Spinacia oleracea |
53000 | - |
SDS-PAGE, cytosolic enzyme | Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Butyl-Toyopearl column chromatography, HiTrap Q column chromatography and Blue Sepharose 6FF column chromatography | Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.056 | - |
cell extract, wild type enzyme | Spinacia oleracea |
13.1 | - |
cell extract, recombinant enzyme | Spinacia oleracea |
24 | - |
after 429fold purification, wild type enzyme | Spinacia oleracea |
100 | - |
after 7.6fold purification, recombinant enzyme | Spinacia oleracea |
Storage Stability | Organism |
---|---|
-80°C, buffer containing 10 mM potassium phosphate at pH 6.5 10 mM 2-mercaptoethanol and 0.1 mM EDTA, several months, no loss of activity | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricyanide + NADPH | - |
Spinacia oleracea | 2 ferrocyanide + NADP+ + H+ | - |
? | |
NADH + 2,6-dichlorophenol indophenol | - |
Spinacia oleracea | NAD+ + ? | - |
? | |
NADH + monodehydroascorbate | - |
Spinacia oleracea | NAD+ + ascorbate | - |
? | |
NADH + monodehydroisoascorbate | - |
Spinacia oleracea | NAD+ + ? | - |
? | |
NADH + p-benzoquinone | - |
Spinacia oleracea | NAD+ + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MDA reductase | - |
Spinacia oleracea |
monodehydroascorbate radical reductase | - |
Spinacia oleracea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | - |
Spinacia oleracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Spinacia oleracea | |
FMN | - |
Spinacia oleracea | |
NADH | - |
Spinacia oleracea |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.095 | - |
dicoumarol | - |
Spinacia oleracea |