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Literature summary for 1.6.5.4 extracted from

  • Yu, S.W.; Kim, Y.R.; Kang, S.O.
    Spectral characterization and chemical modification of FMN-containing ascorbyl free-radical reductase from Pleurotus ostreatus (1999), Biochem. J., 341, 755-763.
No PubMed abstract available
Search for more literature for 1.6.5.4

General Stability

General Stability Organism
freezing without glycerol, complete loss of activity Pleurotus ostreatus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 10 mM, 16% inhibition Pleurotus ostreatus
2,2'-dipyridyl 10 mM, 21% inhibition Pleurotus ostreatus
2,4,6-Trinitrobenzenesulfonic acid 1 mM, 54% inhibition Pleurotus ostreatus
5,5'-dithiobis(2-nitrobenzoic acid) 1 mM, 23% inhibition Pleurotus ostreatus
8-hydroxyquinoline 10 mM, 15% inhibition Pleurotus ostreatus
Cu2+ 0.5 mM, complete inhibition Pleurotus ostreatus
diacetyl 1 mM, 55% inhibition Pleurotus ostreatus
diethyl dicarbonate 0.1 mM, 62% inhibition Pleurotus ostreatus
EDTA 10 mM, 11% inhibition Pleurotus ostreatus
Hg2+ 0.5 mM, complete inhibition Pleurotus ostreatus
iodoacetamide 5 mM, 32% inhibition Pleurotus ostreatus
NaN3 16 mM, 16% inhibition Pleurotus ostreatus
NEM 1 mM, 82% inhibition Pleurotus ostreatus
p-chloromercuribenzoate 0.1 mM, complete inhibition Pleurotus ostreatus
phenyl mercuric acetate 0.1 mM, 85% inhibition Pleurotus ostreatus
Phenylglyoxal 100 mM, 46% inhibition Pleurotus ostreatus
pyridoxal 5'-phosphate 1 mM, 46% inhibition Pleurotus ostreatus
Trinitromethane 10 mM, 11% inhibition Pleurotus ostreatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0021
-
 monodehydroascorbate
-
 Pleurotus ostreatus
0.035
-
 NADH
-
 Pleurotus ostreatus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Pleurotus ostreatus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 2 * 62000, SDS-PAGE Pleurotus ostreatus
127000
-
 gel filtration Pleurotus ostreatus

Organism

Organism UniProt Comment Textmining
Pleurotus ostreatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pleurotus ostreatus

Reaction

Reaction Comment Organism Reaction ID
NADH + H+ + 2 monodehydroascorbate = NAD+ + 2 ascorbate ping pong mechanism Pleurotus ostreatus
a

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Pleurotus ostreatus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.09
-
-
 Pleurotus ostreatus

Storage Stability

Storage Stability Organism
-20°C, 10 mM Tris/HCl buffer, pH 7.4, 2 mM dithiothreitol, 25% glycerol, 1 month, stable Pleurotus ostreatus
4°C, 4°C, in absence of dithiothreitol, complete loss of activity within 1 day Pleurotus ostreatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + 1,4-benzoquinone
-
 Pleurotus ostreatus NAD+ + ?
-
 ?
NADH + 2,6-dichlorophenol indophenol
-
 Pleurotus ostreatus NAD+ + ?
-
 ?
NADH + monodehydroascorbate
-
 Pleurotus ostreatus NAD+ + ascorbate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 62000, SDS-PAGE Pleurotus ostreatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9
-
 Pleurotus ostreatus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 11 stable Pleurotus ostreatus

Cofactor

Cofactor Comment Organism Structure
FMN contains 1 mol of FMN per subunit, flavin is non-covalently bound Pleurotus ostreatus
NADH
-
 Pleurotus ostreatus