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Literature summary for 1.6.5.4 extracted from
- Molecular cloning and characterization of a cDNA encoding pea monodehydroascorbate reductase (1994), J. Biol. Chem., 269, 31129-31133.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli fused to maltose-binding protein | Pisum sativum |
General Stability
| General Stability | Organism |
|---|---|
| 4°C, maltose-binding protein-monodehydroascorbate reductase fusion protein, in presence of 2-mercaptoethanol, 1 month, stable | Pisum sativum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0053 | - |
NADH | maltose-binding protein-monodehydroascorbate reductase fusion protein | Pisum sativum |  |
| 0.0057 | - |
monodehydroascorbate | maltose-binding protein-monodehydroascorbate reductase fusion protein | Pisum sativum | |
| 0.0215 | - |
NADPH | maltose-binding protein-monodehydroascorbate reductase fusion protein | Pisum sativum | |
| 0.052 | - |
oxidized 2,6-dichlorophenolindophenol | maltose-binding protein-monodehydroascorbate reductase fusion protein | Pisum sativum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
x * 47000, SDS-PAGE | Pisum sativum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + monodehydroascorbate | Pisum sativum | key enzyme in maintaining reduced pools of ascorbate | NAD+ + ascorbate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ferricyanide + NADPH + H+ | - |
Pisum sativum | ferrocyanide + NADP+ | - |
r | |
| NADH + H+ + oxidized 2,6-dichlorophenolindophenol | - |
Pisum sativum | NAD+ + reduced 2,6-dichlorophenolindophenol | - |
r | |
| NADH + monodehydroascorbate | - |
Pisum sativum | NAD+ + ascorbate | - |
? | |
| NADH + monodehydroascorbate | key enzyme in maintaining reduced pools of ascorbate | Pisum sativum | NAD+ + ascorbate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 47000, SDS-PAGE | Pisum sativum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | flavin enzyme | Pisum sativum | |
| NADH | - |
Pisum sativum | |
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