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Literature summary for 1.6.5.10 extracted from

  • Barcarolo, M.; Garavaglia, B.; Gottig, N.; Ceccarelli, E.; Catalano-Dupuy, D.; Ottado, J.
    A novel Xanthomonas citri subsp. citri NADPH quinone reductase involved in salt stress response and virulence (2020), Biochim. Biophys. Acta, 1864, 129514 .
    View publication on PubMed
Search for more literature for 1.6.5.10

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 NADPH cosubstrate ferricyanide, pH 6, 30°C Xanthomonas citri

Organism

Organism UniProt Comment Textmining
Xanthomonas citri Q8PKE6
-
-
Xanthomonas citri 306 Q8PKE6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricyanide + NADPH + H+
-
 Xanthomonas citri 2 ferrocyanide + NADP+
-
 ?
2 ferricyanide + NADPH + H+
-
 Xanthomonas citri 306 2 ferrocyanide + NADP+
-
 ?
benzoquinone + NADPH + H+
-
 Xanthomonas citri benzoquinol + NADP+
-
 ?
benzoquinone + NADPH + H+
-
 Xanthomonas citri 306 benzoquinol + NADP+
-
 ?
methyl p-benzoquinone + NADPH + H+
-
 Xanthomonas citri methyl p-benzoquinol + NADP+
-
 ?
methyl p-benzoquinone + NADPH + H+
-
 Xanthomonas citri 306 methyl p-benzoquinol + NADP+
-
 ?
phenyl 1,4-benzoquinone + NADPH + H+
-
 Xanthomonas citri phenyl 1,4-benzoquinol + NADP+
-
 ?
phenyl 1,4-benzoquinone + NADPH + H+
-
 Xanthomonas citri 306 phenyl 1,4-benzoquinol + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
XAC2229
-
 Xanthomonas citri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
884
-
 NADPH cosubstrate ferricyanide, pH 6, 30°C Xanthomonas citri

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Xanthomonas citri
NADH NADH shows a significantly lower reaction than NADPH Xanthomonas citri
NADPH
-
 Xanthomonas citri

Expression

Organism Comment Expression
Xanthomonas citri XAC2229 is expressed under salt stress and during plant-pathogen interaction additional information

General Information

General Information Comment Organism
physiological function Xanthomonas axonopodis pv. citri is the causal agent of citrus canker. Protein XAC2229 has quinone reductase activity using NADPH as an electron donor and also displays diaphorase activity. A deletion mutant shows less cankers and impaired epiphytic survival compared to the wild-type strain. Survival of the deletion mutant is impaired in the presence of H2O2 and it produces more reactive oxygen species and thiobarbituric acid-reactive substances than the wild type strain. Similar results are observed for Escherichia coli expressing the XAC2229 gene Xanthomonas citri