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Literature summary for 1.6.5.10 extracted from

  • Hervas, M.; Lopez-Maury, L.; Leon, P.; Sanchez-Riego, A.M.; Florencio, F.J.; Navarro, J.A.
    ArsH from the cyanobacterium Synechocystis sp. PCC 6803 is an efficient NADPH-dependent quinone reductase (2012), Biochemistry, 51, 1178-1187.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene arsh, expression as N-terminallyy His-tagged enzyme in Escherichia coli strain BL21(DE3) Synechocystis sp.

Inhibitors

Inhibitors Comment Organism Structure
dicoumarol
-
Synechocystis sp.
NADP+ NADP+ acts as a competitive inhibitor for NADPH binding at the active site of an enzyme Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information stopped-flow and steady-state kinetics, overview Synechocystis sp.
0.0012
-
dibromothymoquinone pH 8.0, 25°C, recombinant His-tagged enzyme Synechocystis sp.
0.0013
-
2,5-dimethyl-4-benzoquinone pH 8.0, 25°C, recombinant His-tagged enzyme Synechocystis sp.
0.0028
-
duroquinone pH 8.0, 25°C, recombinant His-tagged enzyme Synechocystis sp.
0.0057
-
menadione pH 8.0, 25°C, recombinant His-tagged enzyme Synechocystis sp.
0.0119
-
coenzyme Q10 pH 8.0, 25°C, recombinant His-tagged enzyme Synechocystis sp.
0.0237
-
2-Hydroxy-1,4-naphthoquinone pH 8.0, 25°C, recombinant His-tagged enzyme Synechocystis sp.
0.0315
-
NADPH pH 8.0, 25°C, recombinant His-tagged enzyme Synechocystis sp.
0.0345
-
anthraquinone-2-sulfonate pH 8.0, 25°C, recombinant His-tagged enzyme Synechocystis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADPH + H+ + a quinone Synechocystis sp. the catalytic cycle of ArsH consists of the acceptance of two electrons from NADPH to reduce the flavin cofactor (reductive half-reaction) and the transfer of these electrons to an acceptor (oxidative half-reaction) NADP+ + a quinol
-
?

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
gene arsH
-

Reaction

Reaction Comment Organism Reaction ID
NADPH + H+ + a quinone = NADP+ + a quinol ping pong reaction mechanism Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information NADH, deazariboflavin, and methylviologen can also act as electron donors Synechocystis sp. ?
-
?
NADPH + H+ + 2,5-dimethyl-4-benzoquinone
-
Synechocystis sp. NADP+ + 2,5-dimethyl-4-benzoquinonl
-
?
NADPH + H+ + 2-hydroxy-1,4-naphthoquinone
-
Synechocystis sp. NADP+ + 2-hydroxy-1,4-naphthoquinol
-
?
NADPH + H+ + a quinone the catalytic cycle of ArsH consists of the acceptance of two electrons from NADPH to reduce the flavin cofactor (reductive half-reaction) and the transfer of these electrons to an acceptor (oxidative half-reaction) Synechocystis sp. NADP+ + a quinol
-
?
NADPH + H+ + anthraquinone-2-sulfonate
-
Synechocystis sp. NADP+ + ?
-
?
NADPH + H+ + coenzyme Q10
-
Synechocystis sp. NADP+ + reduced coenzyme Q10
-
?
NADPH + H+ + dibromothymoquinone
-
Synechocystis sp. NADP+ + dibromothymoquinol
-
?
NADPH + H+ + dichlorophenolindophenol
-
Synechocystis sp. NADP+ + reduced dichlorophenolindophenol
-
?
NADPH + H+ + duroquinone
-
Synechocystis sp. NADP+ + duroquinol
-
?
NADPH + H+ + menadione
-
Synechocystis sp. NADP+ + menadiol
-
?

Synonyms

Synonyms Comment Organism
ArsH
-
Synechocystis sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Synechocystis sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5
-
coenzyme Q10 pH 8.0, 25°C, recombinant His-tagged enzyme Synechocystis sp.
9.4
-
anthraquinone-2-sulfonate pH 8.0, 25°C, recombinant His-tagged enzyme Synechocystis sp.
11
-
2-Hydroxy-1,4-naphthoquinone pH 8.0, 25°C, recombinant His-tagged enzyme Synechocystis sp.
25
-
duroquinone pH 8.0, 25°C, recombinant His-tagged enzyme Synechocystis sp.
26
-
menadione pH 8.0, 25°C, recombinant His-tagged enzyme Synechocystis sp.
35
-
dibromothymoquinone pH 8.0, 25°C, recombinant His-tagged enzyme Synechocystis sp.
36.7
-
2,5-dimethyl-4-benzoquinone pH 8.0, 25°C, recombinant His-tagged enzyme Synechocystis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Synechocystis sp.

Cofactor

Cofactor Comment Organism Structure
FMN
-
Synechocystis sp.

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.008 0.01 with dichlorophenolindophenol, pH 8.0, 25°C, recombinant His-tagged enzyme Synechocystis sp. dicoumarol

General Information

General Information Comment Organism
evolution the enzyme is a member of the family of NADPH-dependent FMN reductases Synechocystis sp.
malfunction arsH mutants are sensitive to the oxidizing agent menadione Synechocystis sp.
additional information although the enzyme is able to stabilize the anionic semiquinone form of the FMN, reduction of quinones involves the hydroquinone form of the flavin cofactor, and the enzymatic reaction occurs through a ping pong-type mechanism. ArsH is able to catalyze one-electron reactions (oxygen and cytocrome c reduction), involving the FMN semiquinone form, but with lower efficiency Synechocystis sp.
physiological function ArsH plays a role in the response to oxidative stress caused by arsenite Synechocystis sp.