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Literature summary for 1.6.3.4 extracted from

  • Li, F.L.; Shi, Y.; Zhang, J.X.; Gao, J.; Zhang, Y.W.
    Cloning, expression, characterization and homology modeling of a novel water-forming NADH oxidase from Streptococcus mutans ATCC 25175 (2018), Int. J. Biol. Macromol., 113, 1073-1079 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptococcus mutans

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling and substrate docking, the nicotinamide ring of NADH extends vertically toward to re-face of coenzyme (FAD) Streptococcus mutans

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 1% residual activity Streptococcus mutans
Li+ 1 mM, 80% residual activity Streptococcus mutans
Mg2+ 1 mM, 85% residual activity Streptococcus mutans
Ni2+ 1 mM, 72% residual activity Streptococcus mutans
Triton X-100
-
Streptococcus mutans
Tween-20
-
Streptococcus mutans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0577
-
NADH pH 7, 25°C Streptococcus mutans

Organism

Organism UniProt Comment Textmining
Streptococcus mutans
-
-
-
Streptococcus mutans ATCC 25175
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
281.2
-
pH 7, 35°C Streptococcus mutans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 NADH + 2 H+ + O2
-
Streptococcus mutans 2 NAD+ + 2 H2O
-
?
2 NADH + 2 H+ + O2
-
Streptococcus mutans ATCC 25175 2 NAD+ + 2 H2O
-
?

Subunits

Subunits Comment Organism
? x * 49900, SDS-PAGE, x * 49930, calculated from sequence Streptococcus mutans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Streptococcus mutans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4 25 72-79% of maximum activity Streptococcus mutans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
3 h, almost 100% residual activity Streptococcus mutans
35
-
3 h, 71% residual activity Streptococcus mutans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Streptococcus mutans

Cofactor

Cofactor Comment Organism Structure
FAD optimum concentration 0.3 mM Streptococcus mutans

pI Value

Organism Comment pI Value Maximum pI Value
Streptococcus mutans calculated from sequence
-
5.4