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Literature summary for 1.6.3.4 extracted from

  • Nowak, C.; Beer, B.; Pick, A.; Roth, T.; Lommes, P.; Sieber, V.
    A water-forming NADH oxidase from Lactobacillus pentosus suitable for the regeneration of synthetic biomimetic cofactors (2018), Front. Microbiol., 6, 957 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.6.3.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Lactiplantibacillus pentosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0179
-
 NADH at pH 7.0 and 37°C Lactiplantibacillus pentosus
1.3
-
 BNAH at pH 7.0 and 37°C Lactiplantibacillus pentosus
1.6
-
 MNAH at pH 7.0 and 37°C Lactiplantibacillus pentosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADH + H+ + O2 Lactiplantibacillus pentosus
-
 NAD+ + H2O
-
 ?
NADH + H+ + O2 Lactiplantibacillus pentosus MP-10
-
 NAD+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus pentosus F6IXY6
-
-
Lactiplantibacillus pentosus MP-10 F6IXY6
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and HiTrap Blue column chromatography Lactiplantibacillus pentosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
BNAH + H+ + O2 i.e. N-benzyl-1,4-dihydronicotinamide Lactiplantibacillus pentosus BNA+ + H2O
-
 ?
BNAH + H+ + O2 i.e. N-benzyl-1,4-dihydronicotinamide Lactiplantibacillus pentosus MP-10 BNA+ + H2O
-
 ?
MNAH + H+ + O2 i.e. N-methyl-1,4-dihydronicotinamide Lactiplantibacillus pentosus MNA+ + H2O
-
 ?
MNAH + H+ + O2 i.e. N-methyl-1,4-dihydronicotinamide Lactiplantibacillus pentosus MP-10 MNA+ + H2O
-
 ?
additional information NADPH can be recognized as a substrate by the enzyme, though the specific activity is only about 2% compared to NADH Lactiplantibacillus pentosus ?
-
 ?
additional information NADPH can be recognized as a substrate by the enzyme, though the specific activity is only about 2% compared to NADH Lactiplantibacillus pentosus MP-10 ?
-
 ?
NADH + H+ + O2
-
 Lactiplantibacillus pentosus NAD+ + H2O
-
 ?
NADH + H+ + O2
-
 Lactiplantibacillus pentosus MP-10 NAD+ + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 51940, calculated from amino acid sequence Lactiplantibacillus pentosus

Synonyms

Synonyms Comment Organism
NOX
-
 Lactiplantibacillus pentosus
water-forming NADH oxidase
-
 Lactiplantibacillus pentosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40
-
 Lactiplantibacillus pentosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.14
-
 MNAH at pH 7.0 and 37°C Lactiplantibacillus pentosus
0.17
-
 BNAH at pH 7.0 and 37°C Lactiplantibacillus pentosus
43.4
-
 NADH at pH 7.0 and 37°C Lactiplantibacillus pentosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Lactiplantibacillus pentosus

pH Range

pH Minimum pH Maximum Comment Organism
6 8 70% activity at pH 6.0 and pH 8.0 Lactiplantibacillus pentosus

Cofactor

Cofactor Comment Organism Structure
FAD contains 13% FAD Lactiplantibacillus pentosus
NADH
-
 Lactiplantibacillus pentosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.09
-
 MNAH at pH 7.0 and 37°C Lactiplantibacillus pentosus
0.13
-
 BNAH at pH 7.0 and 37°C Lactiplantibacillus pentosus