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Literature summary for 1.6.3.4 extracted from

  • Gao, H.; Tiwari, M.K.; Singh, R.K.; Sung, B.H.; Kim, S.C.; Lee, J.K.
    Role of surface residue 184 in the catalytic activity of NADH oxidase from Streptococcus pyogenes (2014), Appl. Microbiol. Biotechnol., 98, 7081-7088 .
    View publication on PubMed
Search for more literature for 1.6.3.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptococcus pyogenes

Protein Variants

Protein Variants Comment Organism
K126R the mutation reduces NADH oxidase activity to about 85% Streptococcus pyogenes
K161R the mutation reduces NADH oxidase activity to about 85% Streptococcus pyogenes
K182R the mutation slightly enhances NADH oxidase activity by approximately 10% Streptococcus pyogenes
K184A the mutant shows strongly reduced activity compared to the wild type enzyme Streptococcus pyogenes
K184D the mutant shows strongly reduced activity compared to the wild type enzyme Streptococcus pyogenes
K184F the mutant shows strongly reduced activity compared to the wild type enzyme Streptococcus pyogenes
K184G the mutant shows strongly reduced activity compared to the wild type enzyme Streptococcus pyogenes
K184Q the mutant shows reduced activity compared to the wild type enzyme Streptococcus pyogenes
K184R the mutation enhances NADH oxidase activity by approximately 50% and improves thermostability with 46.6 % longer half-life at 30°C Streptococcus pyogenes
K241R the mutant shows wild type activity Streptococcus pyogenes
K265R the mutant shows wild type activity Streptococcus pyogenes
K278R the mutant shows wild type activity Streptococcus pyogenes
K374R the mutation reduces NADH oxidase activity to about 50% Streptococcus pyogenes
K393R the mutation reduces NADH oxidase activity to about 70% Streptococcus pyogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0272
-
 NADH wild type enzyme, at pH 7.0 and 55°C Streptococcus pyogenes
0.0274
-
 NADH mutant enzyme K184R, at pH 7.0 and 55°C Streptococcus pyogenes
0.0276
-
 NADH mutant enzyme K184G, at pH 7.0 and 55°C Streptococcus pyogenes
0.0282
-
 NADH mutant enzyme K184Q, at pH 7.0 and 55°C Streptococcus pyogenes
0.0285
-
 NADH mutant enzyme K184F, at pH 7.0 and 55°C Streptococcus pyogenes
0.0296
-
 NADH mutant enzyme K184D, at pH 7.0 and 55°C Streptococcus pyogenes
0.0483
-
 NADH mutant enzyme K184A, at pH 7.0 and 55°C Streptococcus pyogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADH + H+ + O2 Streptococcus pyogenes
-
 NAD+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus pyogenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Streptococcus pyogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + H+ + O2
-
 Streptococcus pyogenes NAD+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
H2O-forming NADH oxidase
-
 Streptococcus pyogenes
nicotinamide adenine dinucleotide oxidase
-
 Streptococcus pyogenes
NOX
-
 Streptococcus pyogenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7
-
-
 Streptococcus pyogenes

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.67
-
 NADH mutant enzyme K184G, at pH 7.0 and 55°C Streptococcus pyogenes
21
-
 NADH mutant enzyme K184D, at pH 7.0 and 55°C Streptococcus pyogenes
23.17
-
 NADH mutant enzyme K184Q, at pH 7.0 and 55°C Streptococcus pyogenes
35.17
-
 NADH mutant enzyme K184F, at pH 7.0 and 55°C Streptococcus pyogenes
36.33
-
 NADH mutant enzyme K184A, at pH 7.0 and 55°C Streptococcus pyogenes
286.7
-
 NADH wild type enzyme, at pH 7.0 and 55°C Streptococcus pyogenes
428.3
-
 NADH mutant enzyme K184R, at pH 7.0 and 55°C Streptococcus pyogenes

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Streptococcus pyogenes

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
241.7
-
 NADH mutant enzyme K184G, at pH 7.0 and 55°C Streptococcus pyogenes
706.7
-
 NADH mutant enzyme K184D, at pH 7.0 and 55°C Streptococcus pyogenes
751.7
-
 NADH mutant enzyme K184A, at pH 7.0 and 55°C Streptococcus pyogenes
1230
-
 NADH mutant enzyme K184F, at pH 7.0 and 55°C Streptococcus pyogenes
8233
-
 NADH mutant enzyme K184Q, at pH 7.0 and 55°C Streptococcus pyogenes
10616
-
 NADH wild type enzyme, at pH 7.0 and 55°C Streptococcus pyogenes
15600
-
 NADH mutant enzyme K184R, at pH 7.0 and 55°C Streptococcus pyogenes