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Literature summary for 1.6.3.3 extracted from

  • Yang, X.; Ma, K.
    Purification and characterization of an NADH oxidase from extremely thermophilic anaerobic bacterium Thermotoga hypogea (2005), Arch. Microbiol., 183, 331-337.
    View publication on PubMed
Search for more literature for 1.6.3.3

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis-(2-nitrobenzoate) 1 mM, 7 min, 50% loss of activity Pseudothermotoga hypogea
CuSO4 1 mM, 1 h at 22°C, 85% remaining activity Pseudothermotoga hypogea
FeSO4 1 mM, 1 h at 22°C, 56% remaining activity Pseudothermotoga hypogea
HgCl2 1 mM, 1 h at 22°C, 6% remaining activity Pseudothermotoga hypogea
MgCl2 1 mM, 1 h at 22°C, 93% remaining activity Pseudothermotoga hypogea
NiCl2 1 mM, 1 h at 22°C, 71% remaining activity Pseudothermotoga hypogea
ZnSO4 1 mM, 1 h at 22°C, 69% remaining activity Pseudothermotoga hypogea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0075
-
 NADH pH 7.0, 80°C Pseudothermotoga hypogea
0.085
-
 O2 pH 7.0, 80°C Pseudothermotoga hypogea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 2 * 50000, SDS-PAGE Pseudothermotoga hypogea
100000
-
 gel filtration Pseudothermotoga hypogea

Organism

Organism UniProt Comment Textmining
Pseudothermotoga hypogea
-
-
-
Pseudothermotoga hypogea DSM 11164
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudothermotoga hypogea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30
-
 pH 7.0, 80°C Pseudothermotoga hypogea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + H+ + O2 the enzyme is highly specific for NADH. No formation of H2O Pseudothermotoga hypogea NAD+ + H2O2
-
 ?
NADH + H+ + O2 the enzyme is highly specific for NADH. No formation of H2O Pseudothermotoga hypogea DSM 11164 NAD+ + H2O2
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 50000, SDS-PAGE Pseudothermotoga hypogea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
 above Pseudothermotoga hypogea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 8 h, no loss of activity Pseudothermotoga hypogea
90
-
 100 min, 50% loss of activity Pseudothermotoga hypogea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Pseudothermotoga hypogea

pH Range

pH Minimum pH Maximum Comment Organism
6.5 7.5 very active in the pH-range 6.5-7.5 Pseudothermotoga hypogea

Cofactor

Cofactor Comment Organism Structure
FAD a flavoprotein, contains 1.8 mol of non-covalently bound FAD per mol of native enzyme Pseudothermotoga hypogea
NADH the enzyme is highly specific for NADH Pseudothermotoga hypogea

General Information

General Information Comment Organism
physiological function further investigation is required to conclude firmly if the purified NADH oxidase is part of an enzyme system that protects anaerobic Thermotoga hypogea from accidental exposure to O2 Pseudothermotoga hypogea