BRENDA - Enzyme Database show
show all sequences of 1.6.3.2

A H2O-producing NADH oxidase from the protozoan parasite Giardia duodenalis

Brown, D.M.; Upcroft, J.A.; Upcroft, P.; Eur. J. Biochem. 241, 155-161 (1996)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
CuSO4
0.2 mM, 97% inhibition
Giardia intestinalis
H2O2
0.5 mM, 50% inhibition
Giardia intestinalis
HgCl2
0.5 mM, 80% inhibition
Giardia intestinalis
additional information
no inhibition by EDTA
Giardia intestinalis
p-chloromercuribenzoate
0.05 mM, complete inhibition
Giardia intestinalis
Quinacrine
1 mM, 73% inhibition
Giardia intestinalis
Quinine
0.2 mM, 50% inhibition
Giardia intestinalis
ZnSO4
0.1 mM, 98% inhibition
Giardia intestinalis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0042
-
NADH
pH 7.8, 22°C
Giardia intestinalis
0.016
-
NADPH
pH 7.8, 22°C
Giardia intestinalis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
activity is not enhanced by the addition of various heavy metals which suggests that the enzyme does not contain any metal ions as co-factors
Giardia intestinalis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45116
-
1 * 45116, calculated from sequence
Giardia intestinalis
46000
-
1 * 46000, SDS-PAGE
Giardia intestinalis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 NADPH + 2 H+ + O2
Giardia intestinalis
the enzyme may be involved in maintenance of an optimum intracellular redox ratio
2 NADP+ + 2 H2O
-
-
?
2 NADPH + 2 H+ + O2
Giardia intestinalis BRIS/83/HEPU/106
the enzyme may be involved in maintenance of an optimum intracellular redox ratio
2 NADP+ + 2 H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Giardia intestinalis
Q7M4I9
-
-
Giardia intestinalis BRIS/83/HEPU/106
Q7M4I9
-
-
Purification (Commentary)
Commentary
Organism
-
Giardia intestinalis
Source Tissue
Source Tissue
Commentary
Organism
Textmining
trophozoite
-
Giardia intestinalis
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 NADH + H+ + O2
NADPH is more efficient as electron donor compared to NADH
722165
Giardia intestinalis
NAD+ + 2 H2O
-
-
-
?
2 NADH + H+ + O2
NADPH is more efficient as electron donor compared to NADH
722165
Giardia intestinalis BRIS/83/HEPU/106
NAD+ + 2 H2O
-
-
-
?
2 NADPH + 2 H+ + O2
the enzyme may be involved in maintenance of an optimum intracellular redox ratio
722165
Giardia intestinalis
2 NADP+ + 2 H2O
-
-
-
?
2 NADPH + 2 H+ + O2
O2 is the most effective electron acceptor, NADH or NADPH are not oxidized anaerobically
722165
Giardia intestinalis
2 NADP+ + 2 H2O
-
-
-
?
2 NADPH + 2 H+ + O2
the enzyme may be involved in maintenance of an optimum intracellular redox ratio
722165
Giardia intestinalis BRIS/83/HEPU/106
2 NADP+ + 2 H2O
-
-
-
?
2 NADPH + 2 H+ + O2
O2 is the most effective electron acceptor, NADH or NADPH are not oxidized anaerobically
722165
Giardia intestinalis BRIS/83/HEPU/106
2 NADP+ + 2 H2O
-
-
-
?
additional information
cytochrome c is not reduced under any assay conditions used
722165
Giardia intestinalis
?
-
-
-
-
additional information
cytochrome c is not reduced under any assay conditions used
722165
Giardia intestinalis BRIS/83/HEPU/106
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 45116, calculated from sequence; 1 * 46000, SDS-PAGE
Giardia intestinalis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
-
Giardia intestinalis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
9
active over the range pH 7-9
Giardia intestinalis
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
FAD is noncoveltly bound, 1 mol of FAD per mol of polypeptide
Giardia intestinalis
NADH
NADPH is more efficient as electron donor compared to NADH
Giardia intestinalis
NADPH
NADPH is more efficient as electron donor compared to NADH
Giardia intestinalis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
FAD is noncoveltly bound, 1 mol of FAD per mol of polypeptide
Giardia intestinalis
NADH
NADPH is more efficient as electron donor compared to NADH
Giardia intestinalis
NADPH
NADPH is more efficient as electron donor compared to NADH
Giardia intestinalis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CuSO4
0.2 mM, 97% inhibition
Giardia intestinalis
H2O2
0.5 mM, 50% inhibition
Giardia intestinalis
HgCl2
0.5 mM, 80% inhibition
Giardia intestinalis
additional information
no inhibition by EDTA
Giardia intestinalis
p-chloromercuribenzoate
0.05 mM, complete inhibition
Giardia intestinalis
Quinacrine
1 mM, 73% inhibition
Giardia intestinalis
Quinine
0.2 mM, 50% inhibition
Giardia intestinalis
ZnSO4
0.1 mM, 98% inhibition
Giardia intestinalis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0042
-
NADH
pH 7.8, 22°C
Giardia intestinalis
0.016
-
NADPH
pH 7.8, 22°C
Giardia intestinalis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
activity is not enhanced by the addition of various heavy metals which suggests that the enzyme does not contain any metal ions as co-factors
Giardia intestinalis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45116
-
1 * 45116, calculated from sequence
Giardia intestinalis
46000
-
1 * 46000, SDS-PAGE
Giardia intestinalis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 NADPH + 2 H+ + O2
Giardia intestinalis
the enzyme may be involved in maintenance of an optimum intracellular redox ratio
2 NADP+ + 2 H2O
-
-
?
2 NADPH + 2 H+ + O2
Giardia intestinalis BRIS/83/HEPU/106
the enzyme may be involved in maintenance of an optimum intracellular redox ratio
2 NADP+ + 2 H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Giardia intestinalis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
trophozoite
-
Giardia intestinalis
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 NADH + H+ + O2
NADPH is more efficient as electron donor compared to NADH
722165
Giardia intestinalis
NAD+ + 2 H2O
-
-
-
?
2 NADH + H+ + O2
NADPH is more efficient as electron donor compared to NADH
722165
Giardia intestinalis BRIS/83/HEPU/106
NAD+ + 2 H2O
-
-
-
?
2 NADPH + 2 H+ + O2
the enzyme may be involved in maintenance of an optimum intracellular redox ratio
722165
Giardia intestinalis
2 NADP+ + 2 H2O
-
-
-
?
2 NADPH + 2 H+ + O2
O2 is the most effective electron acceptor, NADH or NADPH are not oxidized anaerobically
722165
Giardia intestinalis
2 NADP+ + 2 H2O
-
-
-
?
2 NADPH + 2 H+ + O2
the enzyme may be involved in maintenance of an optimum intracellular redox ratio
722165
Giardia intestinalis BRIS/83/HEPU/106
2 NADP+ + 2 H2O
-
-
-
?
2 NADPH + 2 H+ + O2
O2 is the most effective electron acceptor, NADH or NADPH are not oxidized anaerobically
722165
Giardia intestinalis BRIS/83/HEPU/106
2 NADP+ + 2 H2O
-
-
-
?
additional information
cytochrome c is not reduced under any assay conditions used
722165
Giardia intestinalis
?
-
-
-
-
additional information
cytochrome c is not reduced under any assay conditions used
722165
Giardia intestinalis BRIS/83/HEPU/106
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 45116, calculated from sequence; 1 * 46000, SDS-PAGE
Giardia intestinalis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
-
Giardia intestinalis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
9
active over the range pH 7-9
Giardia intestinalis
General Information
General Information
Commentary
Organism
physiological function
the enzyme may be involved in maintenance of an optimum intracellular redox ratio
Giardia intestinalis
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme may be involved in maintenance of an optimum intracellular redox ratio
Giardia intestinalis
Other publictions for EC 1.6.3.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743751
Castillo-Villanueva
Cloning, expression and chara ...
Giardia intestinalis
Protein J.
35
24-33
2016
1
-
1
-
-
-
5
2
-
-
1
2
-
1
-
-
1
-
-
-
-
1
2
1
-
-
-
-
1
-
-
1
-
-
-
1
-
1
1
-
-
-
-
5
-
2
-
-
1
2
-
-
-
1
-
-
-
1
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
742466
Harnvoravongchai
Characterization and gene del ...
Thermococcus kodakarensis
Extremophiles
18
603-616
2014
-
-
-
-
-
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
725576
Nisar
TK1299, a highly thermostable ...
Thermococcus kodakarensis
J. Biosci. Bioeng.
116
39-44
2013
-
-
1
-
-
-
-
1
-
-
3
-
-
1
-
-
1
-
-
-
-
-
2
1
1
1
2
1
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
-
3
-
-
-
-
1
-
-
-
-
2
1
1
1
2
1
1
1
-
-
-
-
-
-
-
-
723187
Jia
An archaeal NADH oxidase cause ...
Thermococcus profundus
Mol. Cells
29
363-371
2010
-
-
1
-
-
-
-
-
-
-
1
2
-
3
-
-
-
-
-
-
-
-
4
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
4
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
722229
Jia
Hexameric ring structure of a ...
Thermococcus profundus
FEBS J.
275
5355-5366
2008
-
-
1
-
3
-
-
2
-
-
2
1
-
4
-
-
1
-
-
-
-
-
3
1
1
1
-
2
1
1
-
3
-
-
-
-
-
1
3
-
3
-
-
-
-
2
-
-
2
1
-
-
-
1
-
-
-
-
3
1
1
1
-
2
1
1
-
-
-
1
1
-
2
2
723205
Li
A likely molecular basis of th ...
Giardia intestinalis
Mol. Microbiol.
59
202-211
2006
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
722165
Brown
A H2O-producing NADH oxidase f ...
Giardia intestinalis, Giardia intestinalis BRIS/83/HEPU/106
Eur. J. Biochem.
241
155-161
1996
-
-
-
-
-
-
8
2
-
1
2
2
-
2
-
-
1
-
-
1
-
-
8
1
-
-
-
-
1
1
-
3
-
-
-
-
-
-
3
-
-
-
-
8
-
2
-
1
2
2
-
-
-
1
-
1
-
-
8
1
-
-
-
-
1
1
-
-
-
1
1
-
-
-