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Literature summary for 1.6.3.1 extracted from

  • Leseney, A.M.; Deme, D.; Legue, O.; Ohayon, R.; Chanson, P.; Sales, J.P.; Pires de Carvalho, D.; Dupuy, C.; Virion, A.
    Biochemical characterization of a Ca2+/NAD(P)H-dependent H2O2 generator in human thyroid tissue (1999), Biochimie, 81, 373-380.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
phosphate
-
Homo sapiens
phosphate
-
Sus scrofa
thyrotropin
-
Homo sapiens
thyrotropin
-
Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
additional information unidentified inhibitor found in 3000 g particulate fraction from patients with Grave's disease Homo sapiens
additional information
-
Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
NADPH pH 7.4, 30°C, 1.5 mM Ca2+ Sus scrofa
0.1
-
NADPH pH 7.4, 30°C, 1.5 mM Ca2+, patients with Grave's disease Homo sapiens
0.1
-
NADH pH 7.4, 30°C, 1.5 mM Ca2+, patients with Grave's disease Homo sapiens
0.2
-
NADH pH 7.4, 30°C, 1.5 mM Ca2+ Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ regulates enzyme activity Homo sapiens
Ca2+ regulates enzyme activity Sus scrofa
Ca2+ no activity without Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD(P)H + H+ + O2 Homo sapiens production of hydrogen peroxide in thyroid gland that is required for thyroid hormone synthesis NAD(P)+ + H2O2
-
?
NAD(P)H + H+ + O2 Sus scrofa production of hydrogen peroxide in thyroid gland that is required for thyroid hormone synthesis NAD(P)+ + H2O2
-
?
NAD(P)H + H+ + O2 Homo sapiens involved in Grave's disease NAD(P)+ + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
female
-
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
thyroid gland
-
Homo sapiens
-
thyroid gland
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.002
-
pH 7.4, 30°C, 1.5 mM Ca2+, saturating concentration of NADPH Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD(P)H + H+ + O2
-
Homo sapiens NAD(P)+ + H2O2
-
?
NAD(P)H + H+ + O2
-
Sus scrofa NAD(P)+ + H2O2
-
?
NAD(P)H + H+ + O2 production of hydrogen peroxide in thyroid gland that is required for thyroid hormone synthesis Homo sapiens NAD(P)+ + H2O2
-
?
NAD(P)H + H+ + O2 production of hydrogen peroxide in thyroid gland that is required for thyroid hormone synthesis Sus scrofa NAD(P)+ + H2O2
-
?
NAD(P)H + H+ + O2 involved in Grave's disease Homo sapiens NAD(P)+ + H2O2
-
?
NADH + H+ + O2
-
Homo sapiens NAD+ + H2O2
-
?
NADH + H+ + O2
-
Sus scrofa NAD+ + H2O2
-
?

Cofactor

Cofactor Comment Organism Structure
FAD
-
Homo sapiens
FAD
-
Sus scrofa
FMN can replace for FAD Homo sapiens
NADH can replace NADPH Homo sapiens
NADPH
-
Homo sapiens
NADPH
-
Sus scrofa