Literature summary for 1.6.2.4 extracted from

Park, H.G.; Lim, Y.R.; Han, S.; Jeong, D.; Kim, D.
Enhanced purification of recombinant rat NADPH-P450 reductase by using a hexahistidine-tag (2017), J. Microbiol. Biotechnol., 27, 983-989 .

Search for more literature for 1.6.2.4

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endoplasmic reticulum	membrane of endoplasmic reticulum	Rattus norvegicus	5783	-
membrane	membrane of endoplasmic reticulum	Rattus norvegicus	16020	-

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	P00388	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein, using Ni2+-affinity chromatography	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 ferricytochrome c + NADPH	-	Rattus norvegicus	2 ferrocytochrome c + NADP+ + H+	-	?
2 oxidized nitroblue tetrazolium + NADPH	-	Rattus norvegicus	2 reduced nitroblue tetrazolium + NADP+ + H+	-	?

Subunits

Subunits	Comment	Organism
?	x * 78000, SDS-PAGE	Rattus norvegicus

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Rattus norvegicus
FMN	-	Rattus norvegicus
NADPH	-	Rattus norvegicus

General Information

General Information	Comment	Organism
physiological function	enzyme successfully supports the catalytic activities of human P450 1A2 and 2A6 and fungal CYP52A21	Rattus norvegicus

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Release 2023.1 (January 2023)