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Literature summary for 1.6.2.4 extracted from

  • Girhard, M.; Tieves, F.; Weber, E.; Smit, M.S.; Urlacher, V.B.
    Cytochrome P450 reductase from Candida apicola: versatile redox partner for bacterial P450s (2013), Appl. Microbiol. Biotechnol., 97, 1625-1635.
    View publication on PubMed
Search for more literature for 1.6.2.4

Cloned(Commentary)

Cloned (Comment) Organism
gene cpr, DNA and amino acid sequence determination and analysis, expression of the N-terminally truncated and His6-tagged enzyme, lacking the putative membrane anchor, in Escherichia coli strain BL21 (DE3) Starmerella apicola

Protein Variants

Protein Variants Comment Organism
additional information truncation f the N-terminal putative membrane anchor leads to increased activity in reducing cytochrome c as compared to the wild-type enzyme, overview Starmerella apicola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Starmerella apicola
0.0138
-
 cytochrome c pH 7.5, 25°C Starmerella apicola

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76068
-
 x * 76068, sequence calculation Starmerella apicola

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADPH + H+ + cytochrome c Starmerella apicola
-
 NADP+ + reduced cytochrome c
-
 ?
NADPH + H+ + cytochrome c Starmerella apicola ATCC 96134
-
 NADP+ + reduced cytochrome c
-
 ?

Organism

Organism UniProt Comment Textmining
Starmerella apicola H2ER12 gene cpr
-
Starmerella apicola ATCC 96134 H2ER12 gene cpr
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally truncated and His6-tagged enzyme, lacking the putative membrane anchor, from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration to over 98% purity Starmerella apicola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADPH + H+ + cytochrome c
-
 Starmerella apicola NADP+ + reduced cytochrome c
-
 ?
NADPH + H+ + cytochrome c
-
 Starmerella apicola ATCC 96134 NADP+ + reduced cytochrome c
-
 ?

Subunits

Subunits Comment Organism
? x * 76068, sequence calculation Starmerella apicola

Synonyms

Synonyms Comment Organism
CPR
-
 Starmerella apicola
cytochrome P450-diflavin reductase
-
 Starmerella apicola

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Starmerella apicola

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
31.92
-
 cytochrome c pH 7.5, 25°C Starmerella apicola

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Starmerella apicola

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Starmerella apicola
FMN
-
 Starmerella apicola
additional information CPR is a diflavin protein containing the flavin cofactors FAD, accepting hydride ions from NADPH, and FMN, accepting single electrons from FAD and reducing the heme center of the P450 monooxygenase domain Starmerella apicola
NADPH
-
 Starmerella apicola

pI Value

Organism Comment pI Value Maximum pI Value
Starmerella apicola sequence calculation
-
 6

General Information

General Information Comment Organism
additional information CPR is a diflavin protein containing the flavin cofactors FAD, accepting hydride ions from NADPH, and FMN, accepting single electrons from FAD and reducing the heme center of the P450 monooxygenase domain Starmerella apicola