Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Gossypium hirsutum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphenyleneiodonium | about 60% inhibition at 0.02 mM and more than 90% inhibition at 0.06 mM; about 60% inhibition at 0.02 mM and more than 90% inhibition at 0.06 mM | Gossypium hirsutum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
ferricytochrome c | isozyme CPR1, at 25°C, pH 7.4 | Gossypium hirsutum | |
0.0016 | - |
ferricytochrome c | isozyme CPR1, at 25°C, pH 7.4 | Gossypium hirsutum | |
0.0046 | - |
NADPH | isozyme CPR1, at 25°C, pH 7.4 | Gossypium hirsutum | |
0.0056 | - |
NADPH | isozyme CPR1, at 25°C, pH 7.4 | Gossypium hirsutum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | isozyme CPR1 increases to about 2old in the presence of 0.1 M KCl, then decreases at higher concentrations of KCl | Gossypium hirsutum | |
K+ | isozyme CPR2 increases to about 2old in the presence of 0.1 M KCl, then decreases at higher concentrations of KCl | Gossypium hirsutum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77140 | - |
isozyme CPR1, calculated from amino acid sequence | Gossypium hirsutum |
78590 | - |
isozyme CPR2, calculated from amino acid sequence | Gossypium hirsutum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gossypium hirsutum | C0LLU4 | cultivars CCRI1 2 and CCRI20 | - |
Gossypium hirsutum | C0LLU5 | cultivars CCRI1 2 and CCRI20 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography | Gossypium hirsutum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.15 | - |
isozyme CPR2, using ferricyanide as cosubstrate, at 25°C, pH 7.4 | Gossypium hirsutum |
3.17 | - |
isozyme CPR2, using ferricytochrome c as cosubstrate, at 25°C, pH 7.4 | Gossypium hirsutum |
3.3 | - |
isozyme CPR2, for NADPH with cytochrome c as cosubstrate, at 25°C, pH 7.4 | Gossypium hirsutum |
3.47 | - |
isozyme CPR1, using ferricyanide as cosubstrate, at 25°C, pH 7.4 | Gossypium hirsutum |
4.69 | - |
isozyme CPR1, using ferricytochrome c as cosubstrate, at 25°C, pH 7.4 | Gossypium hirsutum |
4.78 | - |
isozyme CPR1, for NADPH with cytochrome c as cosubstrate, at 25°C, pH 7.4 | Gossypium hirsutum |
9.32 | - |
isozyme CPR1, using dichlorophenolindophenol as cosubstrate, at 25°C, pH 7.4 | Gossypium hirsutum |
9.84 | - |
isozyme CPR2, using dichlorophenolindophenol as cosubstrate, at 25°C, pH 7.4 | Gossypium hirsutum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricyanide + NADPH | - |
Gossypium hirsutum | 2 ferrocyanide + NADP+ + H+ | - |
? | |
2 ferricytochrome c + NADPH | - |
Gossypium hirsutum | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
2 ferricytochrome P450 + NADPH | - |
Gossypium hirsutum | 2 ferrocytochrome P450 + NADP+ + H+ | - |
? | |
additional information | no activity with NADH | Gossypium hirsutum | ? | - |
? | |
NADPH + H+ + dichlorophenolindophenol | - |
Gossypium hirsutum | NADP+ + reduced dichlorophenolindophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CPR1 | isozyme | Gossypium hirsutum |
CPR2 | isozyme | Gossypium hirsutum |
cytochrome P450 reductase | - |
Gossypium hirsutum |
NADPH: cytochrome P450 reductase | - |
Gossypium hirsutum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.86 | - |
NADPH | isozyme CPR1, at 25°C, pH 7.4 | Gossypium hirsutum | |
3.97 | - |
ferricytochrome c | isozyme CPR1, at 25°C, pH 7.4 | Gossypium hirsutum | |
10.1 | - |
ferricytochrome c | isozyme CPR1, at 25°C, pH 7.4 | Gossypium hirsutum | |
10.2 | - |
NADPH | isozyme CPR1, at 25°C, pH 7.4 | Gossypium hirsutum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | one mol isozyme CPR1 contains 0.87 mol of FAD | Gossypium hirsutum | |
FAD | one mole isozyme CPR2 contains 0.91 mol of FAD | Gossypium hirsutum | |
FMN | one mol isozyme CPR1 contains 0.83 mol of FMN | Gossypium hirsutum | |
FMN | one mole isozyme CPR2 contains 0.86 mol of FMN | Gossypium hirsutum | |
NADPH | - |
Gossypium hirsutum |
Organism | Comment | Expression |
---|---|---|
Gossypium hirsutum | expression of isozyme CPR2, but not CPR1, is inducible by mechanical wounding and elicitation. Isozyme CPR1 is expressed at a relatively constant level throughout the developmental stages investigated (0-40 days post anthesis), with a slight up-regulation at 15 days post anthesis | up |
Gossypium hirsutum | isozyme CPR2 shows a dramatic increase of transcript abundance around 15-20 days post anthesis | up |
General Information | Comment | Organism |
---|---|---|
physiological function | the recombinant enzymes supports the activity of CYP73A25, a cinnamate 4-hydroxylase of cotton | Gossypium hirsutum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
838 | - |
NADPH | isozyme CPR1, at 25°C, pH 7.4 | Gossypium hirsutum | |
1825 | - |
NADPH | isozyme CPR1, at 25°C, pH 7.4 | Gossypium hirsutum | |
3321 | - |
ferricytochrome c | isozyme CPR1, at 25°C, pH 7.4 | Gossypium hirsutum | |
6305 | - |
ferricytochrome c | isozyme CPR1, at 25°C, pH 7.4 | Gossypium hirsutum |