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Literature summary for 1.6.2.4 extracted from

  • Eberle, D.; Ullmann, P.; Werck-Reichhart, D.; Petersen, M.
    cDNA cloning and functional characterisation of CYP98A14 and NADPH:cytochrome P450 reductase from Coleus blumei involved in rosmarinic acid biosynthesis (2009), Plant Mol. Biol., 69, 239-253.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Coleus scutellarioides
expressed in Escherichia coli strain TOP10 and coexpressed with CYP98A14 in Saccharomyces cerevisiae strain INV Sc1 Coleus scutellarioides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
NADPH
-
Coleus scutellarioides
0.05
-
NADPH recombinant yeast microsomal CPR, pH 7.5, 35°C Coleus scutellarioides
0.215
-
NADPH recombinant yeast internal CPR, pH 7.5, 35°C Coleus scutellarioides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78300
-
calculated from amino acid sequence Coleus scutellarioides

Organism

Organism UniProt Comment Textmining
Coleus scutellarioides C0Z3X6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Coleus scutellarioides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.52
-
-
Coleus scutellarioides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricytochrome c + NADPH
-
Coleus scutellarioides 2 ferrocytochrome c + NADP+ + H+
-
?
NADPH + H+ + 2 oxidized cytochrome c
-
Coleus scutellarioides NADP+ + 2 reduced cytochrome c
-
?

Subunits

Subunits Comment Organism
? x * 78300, calculated Coleus scutellarioides

Synonyms

Synonyms Comment Organism
CPR
-
Coleus scutellarioides
NADPH:cytochrome P450 reductase
-
Coleus scutellarioides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Coleus scutellarioides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Coleus scutellarioides

Cofactor

Cofactor Comment Organism Structure
FAD
-
Coleus scutellarioides
FAD the highest increase of 34% is measured after addition of 0.01 mM FAD Coleus scutellarioides
FMN
-
Coleus scutellarioides
NADPH
-
Coleus scutellarioides