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Literature summary for 1.6.2.4 extracted from
- Biochemical characteristics of purified beef liver NADPH-cytochrome P450 reductase (2002), J. Biochem. Mol. Toxicol., 16, 286-297.
General Stability
| General Stability | Organism |
|---|---|
| 20% glycerol stabilizes enzyme | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.048 | - |
ferricytochrome c | pH 7.7 | Bos taurus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Bos taurus | 16020 | - |
| membrane | - |
Ovis aries | 16020 | - |
| microsome | - |
Bos taurus | - |
- |
| microsome | - |
Ovis aries | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 76000 | - |
SDS-PAGE | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricytochrome P450 + NADPH | Bos taurus | component of the cytochrome P450 dependent monooxygenase system | 2 ferrocytochrome P450 + NADP+ + H+ | - |
? | |
| 2 ferricytochrome P450 + NADPH | Ovis aries | component of the cytochrome P450 dependent monooxygenase system | 2 ferrocytochrome P450 + NADP+ + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Ovis aries | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
- |
Ovis aries |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
| lung | - |
Ovis aries | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.5 | - |
purified enzyme with cytochrome c as substrate, pH 7.7 | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricytochrome c + NADPH | - |
Bos taurus | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| 2 ferricytochrome c + NADPH | - |
Ovis aries | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| 2 ferricytochrome P450 + NADPH | component of the cytochrome P450 dependent monooxygenase system | Bos taurus | 2 ferrocytochrome P450 + NADP+ + H+ | - |
? | |
| 2 ferricytochrome P450 + NADPH | component of the cytochrome P450 dependent monooxygenase system | Ovis aries | 2 ferrocytochrome P450 + NADP+ + H+ | - |
? | |
| benzphetamine | demethylation in the presence of added lipid | Bos taurus | ? | - |
? | |
| benzphetamine | demethylation in the presence of added lipid | Ovis aries | ? | - |
? | |
| cocaine | demethylation in the presence of added lipid | Bos taurus | ? | - |
? | |
| cocaine | demethylation in the presence of added lipid | Ovis aries | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADPH-cytochrome P450 reductase | - |
Bos taurus |
| NADPH-cytochrome P450 reductase | - |
Ovis aries |
| NADPH-ferrihemoprotein reductase | - |
Bos taurus |
| NADPH-ferrihemoprotein reductase | - |
Ovis aries |
| NADPH:ferrihemoprotein oxidoreductase | - |
Bos taurus |
| NADPH:ferrihemoprotein oxidoreductase | - |
Ovis aries |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
85% loss of activity within 1 h without added glycerol, 54% loss of activity within 1 h in the presence of 20% glycerol | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 7.8 | - |
Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | together with FMN | Bos taurus | |
| FAD | together with FMN | Ovis aries | |
| FMN | together with FAD | Bos taurus | |
| FMN | together with FAD | Ovis aries | |
| NADPH | - |
Bos taurus | |
| NADPH | - |
Ovis aries | |
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Release 2023.1 (January 2023)
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