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Literature summary for 1.6.2.4 extracted from

  • Crankshaw, D.L.; Hetnarski, K.; Wilkinson, C.F.
    Purification and characterization of NADPH--cytochrome c reductase from the midgut of the southern armyworm (Spodoptera eridania) (1979), Biochem. J., 181, 593-605.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
menadione slight stimulation Spodoptera eridania
additional information increasing ionic strength, increase in activity Rattus norvegicus
additional information increasing ionic strength, increase in activity Spodoptera eridania

Inhibitors

Inhibitors Comment Organism Structure
2'-AMP competitive inhibition Spodoptera eridania
alizarin
-
Spodoptera eridania
Mersalyl
-
Spodoptera eridania
NADP+ competitive inhibition Spodoptera eridania
p-hydroxymercuribenzoate presence of mercaptoethanol or NADPH provides slight protection against inhibition Spodoptera eridania

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0063
-
NADPH
-
Rattus norvegicus
0.013
-
cytochrome c
-
Rattus norvegicus
0.013
-
cytochrome c
-
Spodoptera eridania
0.0131
-
cytochrome c
-
Rattus norvegicus
0.0341
-
NADPH cosubstrate cytochrome c Spodoptera eridania
0.0466
-
cytochrome c cosubstrate NADPH Spodoptera eridania

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Rattus norvegicus
-
-
microsome
-
Spodoptera eridania
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
x * 70000, SDS-PAGE Spodoptera eridania

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
Sprague-Dawley strain
-
Rattus norvegicus Sprague-Dawley
-
Sprague-Dawley strain
-
Spodoptera eridania
-
southern armyworm
-

Purification (Commentary)

Purification (Comment) Organism
column chromatography on Sephadex G-100 and DEAE-cellulose Rattus norvegicus
column chromatography on Sephadex G-100 and DEAE-cellulose Spodoptera eridania

Source Tissue

Source Tissue Comment Organism Textmining
liver phenobarbital-treated animals Rattus norvegicus
-
midgut of larvae, induction of larvae with pentamethylbenzene Spodoptera eridania
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
40.5
-
-
Rattus norvegicus
40.5
-
-
Spodoptera eridania
56.1
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
-15°C Rattus norvegicus
-15°C, more than 1 year Spodoptera eridania
0-4°C, several months Spodoptera eridania

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricytochrome c + NADPH additional electron acceptor: ferricyanide Rattus norvegicus 2 ferrocytochrome c + NADP+ + H+
-
?
2 ferricytochrome c + NADPH additional electron acceptor: ferricyanide Spodoptera eridania 2 ferrocytochrome c + NADP+ + H+
-
?
2 ferricytochrome c + NADPH additional electron acceptor: neotetrazolium chloride Rattus norvegicus 2 ferrocytochrome c + NADP+ + H+
-
?
2 ferricytochrome c + NADPH additional electron acceptor: neotetrazolium chloride Spodoptera eridania 2 ferrocytochrome c + NADP+ + H+
-
?
2 ferricytochrome c + NADPH additional electron acceptor: 2,6-dichlorophenolindophenol Rattus norvegicus 2 ferrocytochrome c + NADP+ + H+
-
?
2 ferricytochrome c + NADPH additional electron acceptor: 2,6-dichlorophenolindophenol Spodoptera eridania 2 ferrocytochrome c + NADP+ + H+
-
?
2 ferricytochrome c + NADPH additional electron acceptor: ferricyanide Rattus norvegicus Sprague-Dawley 2 ferrocytochrome c + NADP+ + H+
-
?
2 ferricytochrome c + NADPH additional electron acceptor: neotetrazolium chloride Rattus norvegicus Sprague-Dawley 2 ferrocytochrome c + NADP+ + H+
-
?
2 ferricytochrome c + NADPH additional electron acceptor: 2,6-dichlorophenolindophenol Rattus norvegicus Sprague-Dawley 2 ferrocytochrome c + NADP+ + H+
-
?
ferricytochrome c + NADPH + H+
-
Spodoptera eridania ferrocytochrome c + NADP+
-
r

Subunits

Subunits Comment Organism
?
-
Rattus norvegicus
? x * 70000, SDS-PAGE Spodoptera eridania

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
36
-
inactivation above Spodoptera eridania
40
-
50% activity Spodoptera eridania

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9
-
Spodoptera eridania

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9
-
Spodoptera eridania

Cofactor

Cofactor Comment Organism Structure
FAD ratio FAD : FMN 1 : 1 Rattus norvegicus
FAD ratio FAD : FMN 1 : 1 Spodoptera eridania
FMN ratio FAD : FMN 1 : 1 Rattus norvegicus
FMN ratio FAD : FMN 1 : 1 Spodoptera eridania
NADPH
-
Rattus norvegicus
NADPH
-
Spodoptera eridania