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Literature summary for 1.6.2.2 extracted from

  • Szilagyi, J.T.; Fussell, K.C.; Wang, Y.; Jan, Y.H.; Mishin, V.; Richardson, J.R.; Heck, D.E.; Yang, S.; Aleksunes, L.M.; Laskin, D.L.; Laskin, J.D.
    Quinone and nitrofurantoin redox cycling by recombinant cytochrome b5 reductase (2018), Toxicol. Appl. Pharmacol., 359, 102-107 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.6.2.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0026
-
 2-hydroxyestradiol pH 7.8, 37°C Homo sapiens
0.0057
-
 9,10-phenanthrenequinone pH 7.8, 37°C Homo sapiens
0.0098
-
 1,4-Naphthoquinone pH 7.8, 37°C Homo sapiens
0.077
-
 2-methyl-1,4-naphthoquinone pH 7.8, 37°C Homo sapiens
0.27
-
 Nitrofurantoin pH 7.8, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-naphthoquinone + O2 + NADH + H+
-
 Homo sapiens ?
-
 ?
1,4-naphthoquinone + O2 + NADH + H+
-
 Homo sapiens ?
-
 ?
2 ferricytochrome b5 + NADPH + H+
-
 Homo sapiens 2 ferrocytochrome b5 + NADP+ + H+
-
 ?
2-hydroxyestradiol + O2 + NADH + H+
-
 Homo sapiens ?
-
 ?
2-methyl-1,4-naphthoquinone + O2 + NADH + H+
-
 Homo sapiens ?
-
 ?
9,10-phenanthrenequinone + O2 + NADH + H+
-
 Homo sapiens ?
-
 ?
nitrofurantoin + O2 + NADH + H+
-
 Homo sapiens ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.003
-
 2-hydroxyestradiol pH 7.8, 37°C Homo sapiens
0.017
-
 Nitrofurantoin pH 7.8, 37°C Homo sapiens
0.058
-
 2-methyl-1,4-naphthoquinone pH 7.8, 37°C Homo sapiens
0.097
-
 9,10-phenanthrenequinone pH 7.8, 37°C Homo sapiens
0.163
-
 1,4-Naphthoquinone pH 7.8, 37°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADH NADH is 5-10 times more active than NADPH in supporting redox cycling Homo sapiens
NADPH NADH is 5-10 times more active than NADPH in supporting redox cycling Homo sapiens

General Information

General Information Comment Organism
metabolism cytochrome b5 reductase mediates redox cycling of a variety of quinones generating superoxide anion, hydrogen peroxide, and, in the presence of transition metals, hydroxyl radicals. Redox cycling activity is oxygen-dependent and preferentially utilizes NADH as a cosubstrate. Quinone redox cycling inhibits reduction of cytochrome b5 by cytochrome b5 reductase under aerobic conditions Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.062
-
 Nitrofurantoin pH 7.8, 37°C Homo sapiens
0.77
-
 2-methyl-1,4-naphthoquinone pH 7.8, 37°C Homo sapiens
1.08
-
 2-hydroxyestradiol pH 7.8, 37°C Homo sapiens
1.67
-
 1,4-Naphthoquinone pH 7.8, 37°C Homo sapiens
16.67
-
 9,10-phenanthrenequinone pH 7.8, 37°C Homo sapiens