Any feedback?
Literature summary for 1.6.2.2 extracted from
- Evaluation of bioreductive activation of anticancer drugs idarubicin and mitomycin C by NADH-cytochrome b5 reductase and cytochrome P450 2B4 (2013), Xenobiotica, 43, 263-275.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Bos taurus | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricytochrome b5 + NADH | Bos taurus | - |
2 ferrocytochrome b5 + NAD+ + H+ | - |
? |  |
| additional information | Bos taurus | poor activity with the anticancer drug mitomycin C, no activity with anticancer drug idarubicin. The quinone antitumor agents are used in the treatment of several human neoplasms | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from liver by anion-exchange chromatography of the detergent solubilized microsomes on two successive DEAE-cellulose columns and an affinity chromatography on adenosine 5'-diphosphate-agarose, to homogeneity | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricytochrome b5 + NADH | - |
Bos taurus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
| additional information | poor activity with the anticancer drug mitomycin C, no activity with anticancer drug idarubicin. The quinone antitumor agents are used in the treatment of several human neoplasms | Bos taurus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADH-cytochrome b5 reductase | - |
Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | 7.5 | assay at | Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | 7.5 | enzyme activity is higher at pH 7.5 compared to pH 6.6 | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Bos taurus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
