Cloned (Comment) | Organism |
---|---|
- |
Physarum polycephalum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
NADH | - |
Physarum polycephalum | |
0.014 | - |
ferricytochrome b5 | - |
Physarum polycephalum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
x * 29000, SDS-PAGE | Physarum polycephalum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Physarum polycephalum | Q1HA49 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Physarum polycephalum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
48.4 | - |
- |
Physarum polycephalum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome b5 + NADH | - |
Physarum polycephalum | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
2 ferricytochrome b5 + NADPH | with NADPH the enzyme shows about 20% of the activity with NADH | Physarum polycephalum | 2 ferrocytochrome b5 + NADP+ + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, SDS-PAGE | Physarum polycephalum |
Synonyms | Comment | Organism |
---|---|---|
NADH-cytochrome b5 reductase | - |
Physarum polycephalum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
30 min, less than 10% loss of activity | Physarum polycephalum |
50 | - |
30 min, 30 min, about 80% loss of activity | Physarum polycephalum |
55 | - |
10 min, complete loss of activity | Physarum polycephalum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
around, recombinant enzyme | Physarum polycephalum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Physarum polycephalum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Physarum polycephalum | calculated from sequence | - |
8.9 |