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Literature summary for 1.6.2.2 extracted from

  • Ikegami, T.; Kameyama, E.; Yamamoto, S.Y.; Minami, Y.; Yubisui, T.
    Structure and properties of the recombinant NADH-cytochrome b5 reductase of Physarum polycephalum (2007), Biosci. Biotechnol. Biochem., 71, 783-790.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Physarum polycephalum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
NADH
-
Physarum polycephalum
0.014
-
ferricytochrome b5
-
Physarum polycephalum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
x * 29000, SDS-PAGE Physarum polycephalum

Organism

Organism UniProt Comment Textmining
Physarum polycephalum Q1HA49
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Physarum polycephalum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
48.4
-
-
Physarum polycephalum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricytochrome b5 + NADH
-
Physarum polycephalum 2 ferrocytochrome b5 + NAD+ + H+
-
?
2 ferricytochrome b5 + NADPH with NADPH the enzyme shows about 20% of the activity with NADH Physarum polycephalum 2 ferrocytochrome b5 + NADP+ + H+
-
?

Subunits

Subunits Comment Organism
? x * 29000, SDS-PAGE Physarum polycephalum

Synonyms

Synonyms Comment Organism
NADH-cytochrome b5 reductase
-
Physarum polycephalum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
30 min, less than 10% loss of activity Physarum polycephalum
50
-
30 min, 30 min, about 80% loss of activity Physarum polycephalum
55
-
10 min, complete loss of activity Physarum polycephalum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
around, recombinant enzyme Physarum polycephalum

Cofactor

Cofactor Comment Organism Structure
FAD
-
Physarum polycephalum

pI Value

Organism Comment pI Value Maximum pI Value
Physarum polycephalum calculated from sequence
-
8.9