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Literature summary for 1.6.2.2 extracted from

  • Barber, M.J.; Quinn, G.B.
    High-level expression in Escherichia coli of the soluble, catalytic domain of rat hepatic cytochrome b5 reductase (1996), Protein Expr. Purif., 8, 41-47.
    View publication on PubMed
Search for more literature for 1.6.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 NADH recombinant enzyme Rattus norvegicus
0.007
-
 ferricyanide recombinant enzyme Rattus norvegicus
0.011
-
 ferricytochrome b5 recombinant enzyme Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 1 * 30000, recombinant enzyme, SDS-PAGE Rattus norvegicus
30588
-
 1 * 30588, recombinant enzyme, MALDI-TOF mass spectrometry Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, affinity chromatography on 5'-ADP-agarose Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1400
-
 recombinant enzyme Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricytochrome b5 + NADH recombinant enzyme, very low activity with NADPH Rattus norvegicus 2 ferrocytochrome b5 + NAD+ + H+
-
 ?
NADH + ferricytochrome b5
-
 Rattus norvegicus NAD+ + H+ + ferrocytochrome b5
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 30000, recombinant enzyme, SDS-PAGE Rattus norvegicus
monomer 1 * 30588, recombinant enzyme, MALDI-TOF mass spectrometry Rattus norvegicus